纯度 | >90% |
种属 | Mouse |
靶点 | CD47 |
Uniprot No | Q61735 |
内毒素 | <0.1EU/ug |
表达宿主 | 293F |
表达区间 | 19- 158 aa |
活性数据 | Measured by its binding ability in a functional ELISA. When Recombinant Mouse SIRP alpha /CD172a Fc Chimera is coated at 2 μg/mL, Recombinant Mouse CD47 binds with an apparent Kd <0.6 nM. Also measured by its ability to antagonize mouse red blood cell adhesion to immobilized Recombinant Mouse SIRP alpha /CD172a Fc Chimera. The ED50 forthis effect is 2.0-8.0 μg/mL. Optimal dilutions should be determined by each laboratory for each application. |
分子量 | 34- 44 kDa |
蛋白标签 | C-His |
缓冲液 | 冻干粉 |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Recombinant Mouse CD47 Protein (Active) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than >90%
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