关键信息
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基因名
PPP1R14A
- 应用
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别名
PPP1R14A;CPI17;PPP1INL;Protein phosphatase 1 regulatory subunit 14A
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96A00
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表达区间
1-147aa
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氨基酸序列
MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL DVEKWIDGRL EELYRGMEAD MPDEINIDEL LELESEEERS RKIQGLLKSC GKPVEDFIQE LLAKLQGLHR QPGLRQPSPS HDGSLSPLQD RARTAHP
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PPP1R14A, a protein phosphatase 1 regulatory subunit, plays a crucial role in the regulation of various cellular processes, including cell cycle progression, muscle contraction, and signal transduction. The gene encoding PPP1R14A is located on chromosome 3 and is known to interact with protein phosphatase 1 (PP1), a key enzyme involved in the dephosphorylation of serine and threonine residues on target proteins, thus modulating their activity and function. Research has demonstrated that the PPP1R14A protein can inhibit PP1 activity in a calcium-dependent manner, suggesting its involvement in calcium signaling pathways. Alterations in its expression or function have been linked to several diseases, including cancer and cardiovascular disorders, making it an important target for therapeutic intervention. Recent advances in recombinant protein technology have facilitated the production and purification of PPP1R14A, allowing for in-depth studies of its structure, function, and regulatory mechanisms. Understanding how PPP1R14A modulates PP1 activity may provide insights into its role in health and disease, paving the way for the development of novel strategies for disease treatment based on the modulation of PP1-mediated signaling pathways. The exploration of PPP1R14A in various biological contexts is essential for harnessing its potential as a biomarker or therapeutic target in disease management.
