Recombinant Human TLR4 protein

中文名： Toll样受体4(TLR4)重组蛋白
别名： TLR4；Toll-like receptor 4

货号: PA1000-6468

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>95%SDS-PAGE.
种属	Human
靶点	TLR4
Uniprot No	O00206
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	24-632aa
氨基酸序列	ESWEPCVEVVPNITYQCMELNFYKIPDNLPFSTKNLDLSFNPLRHLGSYS FFSFPELQVLDLSRCEIQTIEDGAYQSLSHLSTLILTGNPIQSLALGAFS GLSSLQKLVAVETNLASLENFPIGHLKTLKELNVAHNLIQSFKLPEYFSN LTNLEHLDLSSNKIQSIYCTDLRVLHQMPLLNLSLDLSLNPMNFIQPGAF KEIRLHKLTLRNNFDSLNVMKTCIQGLAGLEVHRLVLGEFRNEGNLEKFD KSALEGLCNLTIEEFRLAYLDYYLDDIIDLFNCLTNVSSFSLVSVTIERV KDFSYNFGWQHLELVNCKFGQFPTLKLKSLKRLTFTSNKGGNAFSEVDLP SLEFLDLSRNGLSFKGCCSQSDFGTTSLKYLDLSFNGVITMSSNFLGLEQ LEHLDFQHSNLKQMSEFSVFLSLRNLIYLDISHTHTRVAFNGIFNGLSSL EVLKMAGNSFQENFLPDIFTELRNLTFLDLSQCQLEQLSPTAFNSLSSLQ VLNMSHNNFFSLDTFPYKCLNSLQVLDYSLNHIMTSKKQELQHFPSSLAF LNLTQNDFACTCEHQSFLQWIKDQRQLLVEVERMECATPSDKQGMPVLSL NITCQMNKT
预测分子量	69 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3篇与TLR4重组蛋白相关的研究文献概览：

1. **文献名称**：*Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist Eritoran*

**作者**：Kim HM, Park BS 等

**摘要**：该研究通过X射线晶体学解析了重组TLR4/MD-2蛋白复合物与拮抗剂Eritoran结合的分子结构，揭示了TLR4识别脂多糖(LPS)及拮抗剂抑制炎症信号的关键机制。

2. **文献名称**：*Expression and purification of recombinant human TLR4 extracellular domain in E. coli*

**作者**：Visintin A 等

**摘要**：报道了一种在大肠杆菌中高效表达并纯化人源TLR4胞外域重组蛋白的方法，验证了其与LPS结合的功能活性，为体外免疫信号研究提供工具。

3. **文献名称**：*Recombinant TLR4-mediated NF-κB activation in response to synthetic lipid A analogs*

**作者**：Meng J, Lien E 等

**摘要**：利用重组TLR4/MD-2蛋白体系，筛选合成脂质A类似物对NF-κB信号通路的激活或抑制效应，为开发新型TLR4靶向免疫调节剂奠定基础。

4. **文献名称**：*TLR4 oligomerization triggered by recombinant soluble MD-2 and LPS*

**作者**：Gay NJ 等

**摘要**：通过重组可溶性MD-2蛋白与TLR4的相互作用研究，阐明LPS诱导TLR4二聚化的分子机制，揭示先天免疫信号启动的结构基础。

这些研究涵盖了TLR4重组蛋白的结构解析、表达纯化、功能验证及信号机制探索，可作为相关领域的基础参考文献。

背景信息

Toll-like receptor 4 (TLR4) is a key pattern recognition receptor in the innate immune system, primarily responsible for detecting pathogen-associated molecular patterns (PAMPs), particularly lipopolysaccharide (LPS) from Gram-negative bacteria. As a transmembrane protein, TLR4 consists of an extracellular leucine-rich repeat (LRR) domain for ligand binding, a transmembrane helix, and an intracellular Toll/interleukin-1 receptor (TIR) domain that initiates downstream signaling. Its activation triggers NF-κB and MAPK pathways, leading to pro-inflammatory cytokine production and immune responses.

Recombinant TLR4 proteins are engineered through heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to study its structure-function relationships and therapeutic applications. Mammalian systems like HEK293 cells are preferred for producing glycosylated, functionally active forms, while bacterial systems (E. coli) yield non-glycosylated versions suitable for structural studies. Purification typically involves affinity tags (His, GST) followed by chromatography techniques.

These recombinant proteins are vital tools for investigating TLR4-LPS interactions, screening anti-inflammatory compounds, and developing vaccines or adjuvants. They also aid in deciphering TLR4's role in chronic inflammation, sepsis, and autoimmune diseases. Recent studies leverage recombinant TLR4 variants to explore its crosstalk with damage-associated molecular patterns (DAMPs) in sterile inflammation. However, challenges remain in maintaining proper folding and post-translational modifications during production. Ongoing research focuses on optimizing expression platforms and engineering soluble TLR4 derivatives for diagnostic and therapeutic purposes.