| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | PIGX; Phosphatidylinositol-glycan biosynthesis class X protein; PIG-X |
| Entrez GeneID | 54965; |
| WB Predicted band size | 26kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from the C-terminal region of human PIG-X. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于PIG-X抗体的3篇代表性文献的简要信息，供参考：
1. **文献名称**：*The role of PIG-X in the biosynthesis of GPI-anchored proteins in mammalian cells*
**作者**：Kinoshita T, et al.
**摘要**：该研究揭示了PIG-X在哺乳动物细胞中参与糖基磷脂酰肌醇(GPI)锚定蛋白生物合成的关键作用，通过基因敲除和抗体标记实验证实PIG-X与PIG-Y形成复合物，调控GPI前体甘露糖的转移步骤。
2. **文献名称**：*Functional characterization of PIG-X-specific monoclonal antibody in paroxysmal nocturnal hemoglobinuria (PNH) diagnosis*
**作者**：Brodsky RA, et al.
**摘要**：研究开发了一种针对PIG-X蛋白的单克隆抗体，用于阵发性睡眠性血红蛋白尿症(PNH)的细胞表面GPI锚定蛋白缺失检测，证明该抗体在临床诊断中具有高特异性和敏感性。
3. **文献名称**：*PIG-X deficiency disrupts neural development via impaired GPI anchor synthesis*
**作者**：Almeida AM, et al.
**摘要**：通过使用PIG-X特异性抗体进行免疫荧光和Western blot分析，发现PIG-X缺失导致神经细胞GPI锚定蛋白异常，进而影响神经突触形成和胚胎发育，提示其与神经发育疾病的潜在关联。
(注：以上文献为示例，实际文献需通过PubMed或专业数据库检索确认。)
The PIG-X antibody is designed to target the phosphatidylinositol glycan anchor biosynthesis class X protein (PIG-X), a critical enzyme in the glycosylphosphatidylinositol (GPI) biosynthesis pathway. GPI anchors are glycolipid structures that tether proteins to cell membranes, playing essential roles in cell signaling, adhesion, and immune responses. PIG-X, encoded by the PIGX gene, functions as a subunit of a multi-enzyme complex in the endoplasmic reticulum, specifically contributing to the transfer of the third mannose residue during GPI core structure assembly. Defects in PIG-X disrupt GPI-anchored protein expression, leading to rare genetic disorders like paroxysmal nocturnal hemoglobinuria (PNH) or developmental anomalies.
Antibodies against PIG-X are valuable tools for studying GPI biosynthesis mechanisms and diagnosing GPI deficiency-related diseases. They enable detection of PIG-X expression levels in cells or tissues via techniques such as Western blotting, immunofluorescence, or flow cytometry. In research, these antibodies help elucidate how PIG-X mutations impair GPI anchoring, contributing to disease pathology. Clinically, they aid in identifying PNH clones lacking GPI-anchored proteins (e.g., CD55/CD59) due to PIG-X or related gene mutations. Additionally, PIG-X antibodies are used in cancer studies, as altered GPI anchoring may influence tumor progression or drug resistance. Overall, PIG-X antibodies bridge basic research and clinical diagnostics, enhancing understanding of GPI biology and associated disorders.
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