| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | HIST1H2BC; H2BFL; HIST1H2BE; H2BFH; HIST1H2BF; H2BFG; HIST1H2BG; H2BFA; HIST1H2BI; H2BFK; Histone H2B type 1-C/E/F/G/I; Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; |
| Entrez GeneID | 3017;8339;8343;8344;8346;8347; |
| WB Predicted band size | 18kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from human Histone H2B around the non-acetylation site of K5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Histone H2B抗体的3篇示例参考文献(注：以下为虚构示例，建议通过学术数据库检索真实文献)：
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1. **文献名称**: "A Monoclonal Antibody Specific for Acetylated Histone H2B Reveals Chromatin Remodeling in Gene Activation"
**作者**: Smith J, et al.
**摘要**: 本研究开发了一种针对乙酰化Histone H2B(H2BK5ac)的单克隆抗体，验证了其在染色质免疫沉淀(ChIP)中的应用，并发现该修饰在基因启动子区域的富集与转录激活密切相关。
2. **文献名称**: "Histone H2B Ubiquitination Dynamics Analyzed by a Novel Polyclonal Antibody"
**作者**: Lee S, et al.
**摘要**: 文章报道了一种新型多克隆抗体，可特异性识别泛素化Histone H2B(H2BK120ub)，用于研究DNA损伤修复过程中H2B泛素化的时空动态变化及其与BRCA1通路的关联。
3. **文献名称**: "Cross-Species Reactivity of Anti-Histone H2B Antibodies in Epigenetic Studies"
**作者**: Garcia R, et al.
**摘要**: 通过比较多种物种(小鼠、人类、斑马鱼)的Histone H2B抗体交叉反应性，揭示了该抗体在进化保守表位检测中的可靠性，为跨物种表观遗传分析提供工具支持。
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建议通过 **PubMed** 或 **Google Scholar** 检索关键词如 "Histone H2B antibody application" 或 "H2B antibody specificity" 获取真实文献。
Histone H2B is a core component of nucleosomes, playing a critical role in chromatin structure and epigenetic regulation. As one of the five major histone families (H1. H2A, H2B, H3. H4), H2B forms a heterodimer with H2A, which combines with H3-H4 tetramers to assemble the nucleosome octamer. This structure packages DNA into chromatin, regulating gene expression, DNA replication, and repair. Post-translational modifications (PTMs) of H2B, such as ubiquitination, phosphorylation, and acetylation, influence chromatin dynamics and cellular processes like transcription and apoptosis.
Antibodies targeting Histone H2B are essential tools for studying chromatin biology. They enable detection of H2B in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Specific antibodies can distinguish between unmodified H2B and its modified forms, aiding research into PTM-related mechanisms in development, differentiation, and disease. For example, H2B ubiquitination is linked to DNA damage response and transcriptional elongation, while dysregulation of histone modifiers is implicated in cancer and neurological disorders.
H2B antibodies are typically generated using immunogenic peptides from conserved regions (e.g., the N-terminal tail) or modified residues. Validation includes testing specificity via knockout controls or peptide-blocking assays. Cross-reactivity with other histone variants (e.g., H2B.1. H2B.W) must be considered. These antibodies have broad applications in epigenetics, cancer research, and stem cell studies, providing insights into chromatin remodeling and disease pathogenesis.
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