| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AKT3 |
| Uniprot No | Q9Y243 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-479aa |
| 氨基酸序列 | MSDVTIVKEGWVQKRGKYIKNWRPRYFLLKTDGSFIGYKEKPQDVDLPYP LNNFSVAKCQLMKTERPKPNTFIIRCLQWTTVIERTFHVDTPEEREEWTE AIQAVADRLQRQEEERMNCSPTSQIDNIGEEEMDASTTHHKRKTMNDFDY LKLLGKGTFGKVILVREKASGKYYAMKILKKEVIIAKDEVAHTLTESRVL KNTRHPFLTSLKYSFQTKDRLCFVMEYVNGGELFFHLSRERVFSEDRTRF YGAEIVSALDYLHSGKIVYRDLKLENLMLDKDGHIKITDFGLCKEGITDA ATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQD HEKLFELILMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDAKEIM RHSFFSGVNWQDVYDKKLVPPFKPQVTSETDTRYFDEEFTAQTITITPPE KYDEDGMDCMDNERRPHFPQFSYSASGRE |
| 预测分子量 | 83 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AKT3重组蛋白的3篇代表性文献示例(内容基于公开研究归纳，具体文献建议通过数据库核实)：
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1. **文献名称**: *"AKT3 regulates cell proliferation and migration via protein kinase signaling in cancer"*
**作者**: Stem, J. R., & Roberts, T. M.
**摘要**: 本研究通过重组表达人源AKT3蛋白，分析其磷酸化活性对癌细胞增殖和迁移的影响，揭示了AKT3在肿瘤发生中的独特作用，并验证了其激酶结构域的功能。
2. **文献名称**: *"Expression and purification of active AKT3 kinase for structural studies"*
**作者**: Sasaki, T., et al.
**摘要**: 报道了一种高效重组AKT3蛋白的昆虫细胞表达系统，通过亲和层析和尺寸排阻色谱纯化获得高纯度蛋白，并利用X射线晶体学解析了其激酶域的活性构象。
3. **文献名称**: *"AKT3 deficiency disrupts neuronal development via mTORC1 signaling"*
**作者**: Parker, L. L., & Cantley, L. C.
**摘要**: 利用重组AKT3蛋白进行体外神经元模型实验，证明AKT3通过调控mTORC1通路影响神经突触生长，提示其在神经发育疾病中的潜在机制。
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**注意**：以上为模拟示例，实际文献请通过PubMed、Web of Science或Google Scholar检索关键词(如“AKT3 recombinant protein”、“AKT3 kinase activity”)获取最新数据。
AKT3 (AKT serine/threonine kinase 3), also known as protein kinase B gamma (PKBγ), is a critical component of the PI3K-AKT-mTOR signaling pathway, which regulates essential cellular processes including survival, proliferation, metabolism, and apoptosis. As one of three AKT isoforms (AKT1. AKT2. AKT3), AKT3 shares structural homology with its isoforms, featuring an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a C-terminal regulatory domain. It is activated by phosphorylation following growth factor or insulin stimulation, mediated by PI3K-generated phosphatidylinositol (3.4.5)-trisphosphate (PIP3).
While AKT1 and AKT2 are ubiquitously expressed, AKT3 shows tissue-specific enrichment in the brain, testes, and heart, and is particularly associated with neurological development. Dysregulation of AKT3 has been implicated in cancers (e.g., melanoma, glioblastoma), overgrowth syndromes (e.g., megalencephaly), and neurodevelopmental disorders. For instance, AKT3 gene amplification or activating mutations are linked to enhanced cell survival in tumors, while loss-of-function variants correlate with microcephaly.
Recombinant AKT3 proteins are engineered in vitro using expression systems (e.g., E. coli, mammalian cells) to produce functional or tagged versions (e.g., His-tag, GST-tag) for research applications. These proteins enable mechanistic studies of AKT3’s role in signaling cascades, substrate interactions, and post-translational modifications. They are also used to screen inhibitors targeting AKT3 hyperactivity in cancer or to model pathological mutations. Purified recombinant AKT3 retains kinase activity and can be utilized in enzymatic assays, structural studies, or antibody production. Its availability accelerates drug discovery and functional genomics, offering insights into isoform-specific roles of AKT kinases in health and disease.
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