| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CA12 |
| Uniprot No | O43570 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-301aa |
| 氨基酸序列 | APVNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQVQVCTAAGLS |
| 预测分子量 | 33.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与CA12(碳酸酐酶XII)重组蛋白相关的代表性文献摘要概括(文献名称与作者为虚构示例，实际需根据真实文献调整)：
1. **《重组碳酸酐酶XII的晶体结构及其催化机制研究》**
作者：Smith A, et al.
摘要：通过重组表达纯化人源CA12蛋白，解析其高分辨率晶体结构，揭示其活性位点特征，并探讨其在肿瘤微环境pH调控中的作用，为靶向抑制剂设计提供结构基础。
2. **《CA12重组蛋白在乳腺癌诊断中的潜在生物标志物作用》**
作者：Zhang L, et al.
摘要：利用重组CA12蛋白制备抗体，分析其在乳腺癌患者血清和组织中的表达水平，发现CA12过表达与肿瘤转移相关，提示其作为诊断标记物的潜力。
3. **《基于重组CA12的高通量药物筛选平台开发》**
作者：Johnson R, et al.
摘要：建立重组CA12蛋白的体外酶活性检测体系，筛选天然化合物库，发现多个新型选择性抑制剂，为治疗CA12相关癌症提供先导化合物。
4. **《CA12重组蛋白在缺氧诱导的肾癌细胞侵袭中的作用》**
作者：Wang Y, et al.
摘要：通过体外重组CA12蛋白功能实验，证明其在缺氧条件下通过调节细胞外pH促进肾癌细胞迁移，为靶向肿瘤代谢治疗提供依据。
**注**：以上为示例性内容，实际文献需通过PubMed、Web of Science等平台检索关键词“Carbonic Anhydrase XII recombinant”或“CA12 recombinant protein”获取准确信息。
Carbonic anhydrase XII (CA12) is a zinc-containing metalloenzyme belonging to the carbonic anhydrase family, which catalyzes the reversible hydration of carbon dioxide to bicarbonate and protons. This enzyme plays a critical role in pH regulation, ion transport, and cellular homeostasis. CA12 is a membrane-associated protein primarily expressed in normal tissues such as the kidneys, colon, and reproductive organs. Its overexpression has been linked to various cancers, including breast, lung, and renal cell carcinomas, where it contributes to tumor microenvironment acidification, promoting invasion, metastasis, and chemoresistance.
The recombinant CA12 protein is engineered through genetic cloning, typically using mammalian or bacterial expression systems, to produce purified, bioactive forms for research and therapeutic applications. Structurally, the recombinant protein retains key functional domains, including the catalytic site and transmembrane region (if expressed as a full-length variant). Researchers utilize it to study CA12's enzymatic activity, pH-regulatory mechanisms, and interactions with inhibitors like acetazolamide. Its role in cancer progression has spurred interest in developing CA12-targeted therapies, including monoclonal antibodies and small-molecule inhibitors.
Additionally, recombinant CA12 serves as an antigen for antibody production in diagnostic assays, aiding in biomarker discovery for cancer detection and prognosis. Studies also explore its involvement in non-cancer pathologies, such as glaucoma and metabolic disorders, broadening its biomedical relevance. The protein’s stability and functional consistency in recombinant form make it a valuable tool for deciphering CA12 biology and advancing translational applications.
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