Recombinant Human YWHAG protein

中文名： 酪氨酸3-单加氧酶激活蛋白γ（YWHAG）重组蛋白
别名： 14-3-3GAMMA；PPP1R170；EIEE56

货号: PA1000-6DB

Price： ￥1380

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	＞90% by SDS-PAGE.
种属	Human
靶点	YWHAG
Uniprot No	P61981
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-247aa
氨基酸序列	MVDREQLVQK ARLAEQAERY DDMAAAMKNV TELNEPLSNE ERNLLSVAYK NVVGARRSSW RVISSIEQKT SADGNEKKIE MVRAYREKIE KELEAVCQDV LSLLDNYLIK NCSETQYESK VFYLKMKGDY YRYLAEVATG EKRATVVESS EKAYSEAHEI SKEHMQPTHP IRLGLALNYS VFYYEIQNAP EQACHLAKTA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDD DGGEGNN
预测分子量	28 kDa
蛋白标签	C-his
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于YWHAG重组蛋白的3篇示例参考文献(注：以下文献为模拟内容，实际文献需通过学术数据库检索确认)：

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1. **文献名称**：*Expression and Functional Characterization of Recombinant YWHAG in Cancer Cell Signaling*

**作者**：Chen L. et al.

**摘要**：本研究成功构建了YWHAG重组蛋白的原核表达系统，并通过亲和层析纯化获得高纯度蛋白。功能实验表明，重组YWHAG通过结合磷酸化Bax蛋白调控线粒体凋亡通路，增强肿瘤细胞对化疗药物的敏感性。

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2. **文献名称**：*Structural Insights into YWHAG-14-3-3γ Interaction with Phosphorylated Client Proteins*

**作者**：Smith J.R., Patel R.

**摘要**：通过X射线晶体学解析了重组YWHAG蛋白与磷酸化p53肽段的复合物结构，揭示了其保守结合口袋的关键氨基酸残基。该研究为设计靶向YWHAG相互作用的药物提供了结构基础。

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3. **文献名称**：*YWHAG Recombinant Protein Attenuates Neurodegeneration in a Mouse Model of Alzheimer’s Disease*

**作者**：Kim S. et al.

**摘要**：利用哺乳动物细胞表达系统制备重组YWHAG蛋白，发现其通过稳定tau蛋白磷酸化水平减少神经元损伤。动物实验显示，脑内注射YWHAG可改善阿尔茨海默病模型小鼠的认知功能。

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**研究方向扩展**：

YWHAG重组蛋白的研究多聚焦于其作为分子支架的相互作用机制(如与凋亡、代谢或神经退行性疾病相关蛋白的结合)，以及其在疾病治疗中的潜在应用。建议通过PubMed或Web of Science以“YWHAG recombinant protein”、“14-3-3 gamma purification”为关键词检索最新文献。

背景信息

**Background of YWHAG Recombinant Protein**

YWHAG (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma), also known as 14-3-3γ, is a member of the highly conserved 14-3-3 protein family. These proteins function as regulatory hubs in eukaryotic cells, modulating signal transduction by binding to phosphorylated serine/threonine residues on client proteins. The 14-3-3γ isoform plays critical roles in diverse cellular processes, including cell cycle regulation, apoptosis, metabolism, and stress responses, by interacting with kinases, phosphatases, transcription factors, and disease-associated targets like CDC25. BAD, and Raf family members.

Recombinant YWHAG protein is engineered *in vitro* using expression systems such as *E. coli* or mammalian cell lines, often fused with tags (e.g., GST, His) to facilitate purification and detection. Its structure features a conserved amphipathic groove that binds phosphorylated motifs, enabling dimerization (homodimers or heterodimers with other 14-3-3 isoforms) essential for scaffolding functions.

This recombinant protein is widely utilized to study 14-3-3γ-mediated interactions *in vitro* (e.g., pull-down assays, co-immunoprecipitation) and serves as a standard in quantitative analyses (e.g., ELISA). It also aids in drug discovery, particularly in screening compounds targeting dysregulated 14-3-3 interactions implicated in cancers, neurodegenerative disorders (e.g., Alzheimer’s), and metabolic diseases. Dysregulation of YWHAG expression or function has been linked to tumor progression and neuronal death, underscoring its therapeutic relevance. By providing a controllable and pure protein source, recombinant YWHAG accelerates mechanistic studies and the development of precision therapies.