纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | CDH3 |
Uniprot No | P22223 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 108-829aa |
氨基酸序列 | DWVVAPISVPENGKGPFPQRLNQLKSNKDRDTKIFYSITGPGADSPPEGVFAVEKETGWLLLNKPLDREEIAKYELFGHAVSENGASVEDPMNISIIVTDQNDHKPKFTQDTFRGSVLEGVLPGTSVMQVTATDEDDAIYTYNGVVAYSIHSQEPKDPHDLMFTIHRSTGTISVISSGLDREKVPEYTLTIQATDMDGDGSTTTAVAVVEILDANDNAPMFDPQKYEAHVPENAVGHEVQRLTVTDLDAPNSPAWRATYLIMGGDDGDHFTITTHPESNQGILTTRKGLDFEAKNQHTLYVEVTNEAPFVLKLPTSTATIVVHVEDVNEAPVFVPPSKVVEVQEGIPTGEPVCVYTAEDPDKENQKISYRILRDPAGWLAMDPDSGQVTAVGTLDREDEQFVRNNIYEVMVLAMDNGSPPTTGTGTLLLTLIDVNDHGPVPEPRQITICNQSPVRQVLNITDKDLSPHTSPFQAQLTDDSDIYWTAEVNEEGDTVVLSLKKFLKQDTYDVHLSLSDHGNKEQLTVIRATVCDCHGHVETCPGPWKGGFILPVLGAVLALLFLLLVLLLLVRKKRKIKEPLLLPEDDTRDNVFYYGEEGGGEEDQDYDITQLHRGLEARPEVVLRNDVAPTIIPTPMYRPRPANPDEIGNFIIENLKAANTDPTAPPYDTLLVFDYEGSGSDAASLSSLTSSASDQDQDYDYLNEWGSRFKKLADMYGGGEDD |
预测分子量 | kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDH3重组蛋白的模拟参考文献示例(内容为虚构,供参考):
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1. **文献名称**: *Recombinant CDH3 Expression and Its Role in Cell Adhesion*
**作者**: Smith A, et al.
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化了CDH3重组蛋白,证实其具有钙依赖性细胞粘附功能,并揭示其在上皮细胞极性形成中的关键作用。
2. **文献名称**: *CDH3 as a Biomarker in Pancreatic Cancer: Recombinant Protein-Based Analysis*
**作者**: Lee J, et al.
**摘要**: 利用重组CDH3蛋白开发ELISA检测方法,发现胰腺癌患者血清中CDH3水平显著升高,提示其作为潜在诊断标志物的价值。
3. **文献名称**: *Structural Characterization of Recombinant CDH3 Extracellular Domain*
**作者**: García R, et al.
**摘要**: 通过X射线晶体学解析CDH3重组胞外域结构,阐明其钙离子结合位点及同源二聚化机制,为靶向药物设计提供依据。
4. **文献名称**: *CDH3 Recombinant Protein Enhances Metastasis in Breast Cancer Models*
**作者**: Chen L, et al.
**摘要**: 体外实验表明,添加重组CDH3蛋白可促进乳腺癌细胞迁移和侵袭,提示其在肿瘤转移中的促癌功能。
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**注意**:以上文献为示例性内容,实际研究请通过PubMed、Google Scholar等平台检索关键词“CDH3 recombinant protein”或“CDH3 cadherin-3”获取真实文献。
CDH3 (Cadherin-3), also known as P-cadherin, is a calcium-dependent cell adhesion protein belonging to the cadherin superfamily. It plays a critical role in maintaining tissue architecture by mediating homophilic cell-cell interactions, primarily in epithelial and placental tissues. Structurally, CDH3 consists of an extracellular domain with five cadherin repeats, a transmembrane domain, and a highly conserved cytoplasmic domain that interacts with catenins to link the cadherin-catenin complex to the actin cytoskeleton. This interaction is essential for cellular polarity, differentiation, and signal transduction.
The recombinant CDH3 protein is engineered through molecular cloning techniques, typically expressed in mammalian or insect cell systems to ensure proper post-translational modifications, such as glycosylation and calcium-binding capacity. Recombinant CDH3 retains the functional extracellular domain, enabling its use in studying cell adhesion mechanisms, epithelial-mesenchymal transition (EMT), and tissue development. Its dysregulation has been implicated in pathological conditions, including cancer progression and metastasis, where overexpression of CDH3 correlates with aggressive tumor behavior in breast, gastric, and ovarian cancers. Researchers utilize recombinant CDH3 to investigate its role in tumor microenvironment interactions, biomarker potential, and therapeutic targeting. Additionally, it serves as a tool for antibody production, drug screening, and structural studies to decipher cadherin-mediated signaling pathways. The availability of recombinant CDH3 has significantly advanced both basic and translational research in developmental biology, oncology, and regenerative medicine.
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