Recombinant Human BPNT1 protein

中文名： 3’,5’-二磷酸核苷酸酶1(BPNT1)重组蛋白
别名： BPNT1；3'(2'),5'-bisphosphate nucleotidase 1

货号: PA1000-363DB

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	> 90 % SDS-PAGE.
种属	Human
靶点	BPNT1
Uniprot No	O95861
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-308aa
氨基酸序列	MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMASSNTVLMRLVAS AYSIAQKAGMIVRRVIAEGDLGIVEKTCATDLQTKADRLAQMSICSSLAR KFPKLTIIGEEDLPSEEVDQELIEDSQWEEILKQPCPSQYSAIKEEDLVV WVDPLDGTKEYTEGLLDNVTVLIGIAYEGKAIAGVINQPYYNYEAGPDAV LGRTIWGVLGLGAFGFQLKEVPAGKHIITTTRSHSNKLVTDCVAAMNPDA VLRVGGAGNKIIQLIEGKASAYVFASPGCKKWDTCAPEVILHAVGGKLTD IHGNVLQYHKDVKHMNSAGVLATLRNYDYYASRVPESIKNALVP
预测分子量	38 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于BPNT1重组蛋白的假设性参考文献示例(仅供参考，具体文献需通过学术数据库查询)：

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1. **标题**：*Recombinant Expression and Functional Characterization of Human BPNT1 in a Mammalian System*

**作者**：Smith, J., et al.

**摘要**：本研究利用HEK293细胞成功表达并纯化了重组人源BPNT1蛋白，分析了其酶动力学特性，证实其对3'-磷酸腺苷5'-磷酸盐(PAP)的特异性水解活性，为研究其在核苷酸代谢中的作用提供了工具。

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2. **标题**：*Structural Insights into BPNT1 Substrate Specificity through Crystallographic Analysis*

**作者**：Lee, S., et al.

**摘要**：通过X射线晶体学解析了重组BPNT1蛋白的三维结构，揭示了其底物结合口袋的关键氨基酸残基，阐明了BPNT1选择性催化磷酸水解的分子机制。

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3. **标题**：*BPNT1 Knockdown and Recombinant Protein Rescue in a Zebrafish Model of Sulfation Deficiency*

**作者**：Garcia, R., et al.

**摘要**：在斑马鱼模型中，通过重组BPNT1蛋白回补实验验证了其调节硫酸化代谢通路的功能，证明BPNT1缺失导致软骨发育异常，而外源重组蛋白可部分恢复表型。

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4. **标题**：*High-Yield Production of BPNT1 in E. coli and Its Application in Phosphate Homeostasis Studies*

**作者**：Wang, Y., et al.

**摘要**：开发了一种基于大肠杆菌的高效重组BPNT1表达纯化方案，并利用该蛋白证明其在细胞磷酸盐稳态调控中的关键作用，为代谢疾病研究提供实验基础。

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**备注**：以上为模拟参考文献，实际文献需通过PubMed、Web of Science等平台搜索关键词(如“BPNT1 recombinant”“BPNT1 expression”“BPNT1 phosphatase”)获取。

背景信息

BPNT1 (3'(2'),5'-Bisphosphate Nucleotidase 1), also known as BPNT-1 or SAL1. is a key enzyme involved in cellular phosphate metabolism. It specifically hydrolyzes 3'-phosphoadenosine 5'-phosphate (PAP) and 3'-phosphoadenosine 5'-phosphosulfate (PAPS), converting them into adenosine monophosphate (AMP) and inorganic phosphate/sulfate. This reaction is critical for regulating sulfur metabolism, nucleotide homeostasis, and maintaining cellular redox balance. BPNT1's activity prevents the toxic accumulation of PAP and PAPS, which can inhibit sulfotransferases and RNA-processing enzymes, thereby influencing processes like sulfation reactions, RNA splicing, and stress response pathways.

Recombinant BPNT1 protein is commonly produced in heterologous expression systems (e.g., E. coli or mammalian cells) for functional and structural studies. The protein typically retains conserved catalytic residues (e.g., Cys53. His76. and His77 in humans) essential for its phosphatase activity. Its structure features a haloacid dehalogenase (HAD)-like fold, characteristic of the two-domain phosphatase superfamily. Researchers often use affinity tags (His-tag, GST-tag) during purification to facilitate isolation and characterization.

Studies using recombinant BPNT1 have revealed its role in metabolic disorders, including hepatic dysfunction and neurological conditions. For instance, BPNT1 mutations are linked to childhood-onset brain calcification and developmental delays. In cancer biology, altered BPNT1 expression correlates with tumor progression through mechanisms involving oxidative stress regulation. Current research focuses on its potential as a therapeutic target and its interactions with other metabolic enzymes. The recombinant protein serves as a vital tool for enzymatic assays, inhibitor screening, and deciphering molecular mechanisms in sulfur metabolism pathways.