| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Myosin light chain kinase 3, Cardiac-MyBP-C-associated Ca/CaM kinase, Cardiac-MLCK, Mylk3 |
| Entrez GeneID | 213435 |
| WB Predicted band size | 86.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Mylk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-203 amino acids from the N-terminal region of mouse Mylk3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse MYLK3 (N-term)抗体的假设性参考文献示例，供参考：
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1. **文献名称**: *"Characterization of MYLK3 in Cardiac Muscle Development"*
**作者**: Smith A, et al. (2018)
**摘要**: 本研究通过Western blot和免疫组化分析了小鼠心脏组织中MYLK3的表达模式，使用Mouse MYLK3 (N-term)抗体验证了其N端特异性结合。结果提示MYLK3在胚胎期心肌细胞分化中起关键作用。
2. **文献名称**: *"Role of MYLK3 in Smooth Muscle Contractility"*
**作者**: Johnson B, et al. (2020)
**摘要**: 文章利用Mouse MYLK3 (N-term)抗体进行免疫荧光和蛋白质印迹，证实MYLK3在小鼠肠道平滑肌中的高表达，并发现其磷酸化活性与钙离子信号通路相关，影响收缩功能。
3. **文献名称**: *"MYLK3 Knockout Mouse Model and Phenotypic Analysis"*
**作者**: Lee C, et al. (2019)
**摘要**: 研究构建了MYLK3基因敲除小鼠，通过该抗体检测到N端蛋白缺失，发现敲除小鼠出现心脏肥大和运动耐力下降，提示MYLK3对维持正常心脏功能至关重要。
4. **文献名称**: *"MYLK3 Expression in Tumor Metastasis"*
**作者**: Chen D, et al. (2021)
**摘要**: 使用Mouse MYLK3 (N-term)抗体对小鼠肿瘤组织进行免疫组化分析，发现高表达MYLK3的癌细胞侵袭性增强，可能与上皮-间质转化(EMT)通路激活有关。
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**注**：以上为模拟文献，实际引用需查询PubMed、Google Scholar等数据库获取真实研究。
The Mouse Mylk3 (N-term) antibody is a monoclonal or polyclonal antibody designed to specifically detect the N-terminal region of myosin light chain kinase 3 (MYLK3) in mouse-derived samples. MYLK3 belongs to the myosin light chain kinase family, which regulates smooth muscle and non-muscle cell contraction by phosphorylating myosin regulatory light chains. This enzyme plays a critical role in cellular processes such as cytokinesis, motility, and vascular tone regulation. The N-terminal region of MYLK3 is essential for its catalytic activity and interaction with binding partners, making antibodies targeting this domain valuable tools for studying the protein's expression, localization, and functional mechanisms.
Research applications of this antibody include Western blotting, immunohistochemistry, and immunofluorescence to investigate MYLK3's role in physiological and pathological contexts, such as cardiovascular development, smooth muscle dysfunction, or cancer progression. Its specificity for the N-terminal epitope helps distinguish MYLK3 from other isoforms (e.g., MYLK1 or MYLK2), ensuring accurate detection in complex biological samples. Validation typically involves testing reactivity in MYLK3-knockout models or overexpression systems to confirm minimal cross-reactivity. As MYLK3 mutations have been linked to congenital heart defects, this antibody also supports studies exploring genetic disorders and therapeutic targets.
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