| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 1/10-1/50 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Putative methyl-CpG-binding domain protein 3-like 3, MBD3-like protein 3, MBD3L3 |
| Entrez GeneID | 653657 |
| WB Predicted band size | 23.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MBD3L3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-184 amino acids from the C-terminal region of human MBD3L3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MBD3L3抗体的示例性参考文献(注：由于MBD3L3研究较为小众，以下内容为模拟生成，建议通过PubMed或专业数据库查询真实文献)：
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1. **文献名称**: "Characterization of a Novel Monoclonal Antibody Against MBD3L3 for Epigenetic Studies"
**作者**: Zhang Y, et al.
**摘要**: 本研究开发了一种针对MBD3L3蛋白的单克隆抗体，通过Western blot和免疫荧光验证其特异性。实验表明该抗体可识别内源性MBD3L3.且在DNA甲基化依赖性染色质重塑复合物中检测到其相互作用，为表观遗传调控研究提供了工具。
2. **文献名称**: "MBD3L3 Antibody Application in Neural Stem Cell Differentiation"
**作者**: Lee S, et al.
**摘要**: 利用MBD3L3抗体探究其在神经干细胞分化中的作用。免疫组化显示MBD3L3在分化后期表达上调，ChIP-seq分析揭示其通过结合甲基化DNA区域抑制特定基因转录，提示其在神经发育中的调控功能。
3. **文献名称**: "Development of a Polyclonal Antibody for MBD3L3 and Its Role in Cancer Biomarker Screening"
**作者**: Gupta R, et al.
**摘要**: 报道了一种兔源多克隆抗体的制备，并在多种癌细胞系中验证其应用。研究发现MBD3L3在结肠癌中表达异常，可能作为潜在表观遗传生物标志物，抗体为临床样本分析提供了技术支持。
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**建议**：若需真实文献，可通过以下方式获取：
1. 在PubMed中输入关键词 "MBD3L3 antibody" 或 "MBD3L3 immune localization"。
2. 查阅抗体供应商(如Abcam、CST)的产品说明书引用文献。
3. 关注表观遗传学或DNA甲基化相关研究，MBD3L3可能作为复合物成员被提及。
The MBD3L3 (Methyl-CpG Binding Domain Protein 3-Like 3) antibody is a tool used to study the MBD3L3 protein, a member of the methyl-CpG-binding domain (MBD) family. Unlike canonical MBD proteins (e.g., MBD1-4) that bind methylated DNA and recruit chromatin-modifying complexes, MBD3L3 lacks a functional MBD domain, suggesting divergent roles. It is closely related to MBD3L1 and MBD3L2. forming a subgroup within the MBD3-like family. MBD3L3 is implicated in transcriptional regulation, potentially acting as a co-factor for the NuRD (Nucleosome Remodeling and Deacetylase) complex, which modulates chromatin structure and gene expression. Its expression is tissue-specific, with higher levels observed in germ cells and certain cancers, linking it to developmental processes and oncogenesis.
Antibodies targeting MBD3L3 are typically raised in rabbits or mice using immunogenic peptides or recombinant protein fragments. They enable detection via techniques like Western blotting, immunohistochemistry, or immunofluorescence, aiding in elucidating MBD3L3’s localization, interaction partners, and mechanistic roles. Research utilizing these antibodies has explored MBD3L3’s involvement in DNA demethylation dynamics, pluripotency in stem cells, and epigenetic dysregulation in malignancies. However, its precise biological functions remain under investigation, partly due to overlapping roles with other MBD3-like proteins. Validated MBD3L3 antibodies are critical for distinguishing its unique contributions to epigenetic pathways and disease contexts.
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