| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 1/10-1/50 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | C-1-tetrahydrofolate synthase, cytoplasmic, C1-THF synthase, Methylenetetrahydrofolate dehydrogenase, Methenyltetrahydrofolate cyclohydrolase, Formyltetrahydrofolate synthetase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, MTHFD1, MTHFC, MTHFD |
| Entrez GeneID | 4522 |
| WB Predicted band size | 101.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MTHFD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 535-562 amino acids from the Central region of human MTHFD1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于MTHFD1(Center P550)抗体的3篇参考文献示例：
1. **文献名称**：*MTHFD1 regulates mitochondrial folate metabolism and colorectal cancer progression*
**作者**：Smith A, et al.
**摘要**：本研究利用针对MTHFD1中心区域(P550表位)的特异性抗体，通过免疫印迹和免疫组化分析结直肠癌组织中MTHFD1的表达水平，发现其高表达与线粒体叶酸代谢异常及肿瘤侵袭性相关。
2. **文献名称**：*Genetic and functional analysis of MTHFD1 in neural tube defects*
**作者**：Chen L, et al.
**摘要**：通过Western blot和免疫荧光技术，使用抗MTHFD1(P550)抗体检测小鼠胚胎神经管组织中MTHFD1的亚细胞定位，揭示其叶酸代谢功能缺陷与神经管畸形的关联。
3. **文献名称**：*Targeting MTHFD1 in cardiovascular disease: Insights from a mouse model*
**作者**：Wang Y, et al.
**摘要**：研究采用针对MTHFD1中心区域的抗体(P550表位)分析动脉粥样硬化小鼠模型的血管组织，证实MTHFD1表达下调导致同型半胱氨酸累积，促进血管内皮损伤。
(注：以上文献信息为模拟示例，实际引用需根据具体文献调整。)
The MTHFD1 (Center P550) antibody detects a central region of methylenetetrahydrofolate dehydrogenase, cyclohydrolase, and formyltetrahydrofolate synthetase 1 (MTHFD1), a bifunctional enzyme critical in one-carbon metabolism. MTHFD1 facilitates the interconversion of folate derivatives to generate precursors for nucleotide synthesis (purines, thymidylate) and methyl group donors (e.g., S-adenosylmethionine). The enzyme contains three functional domains: a dehydrogenase, cyclohydrolase, and synthetase. The "Center P550" epitope likely resides within the synthetase domain, which is involved in formyltetrahydrofolate production.
This rabbit polyclonal antibody, raised against a peptide near position P550. is commonly used in Western blotting (WB), immunohistochemistry (IHC), and immunocytochemistry (ICC) to study MTHFD1 expression and localization. It is validated for specificity in human and mouse tissues, often showing cytoplasmic staining, consistent with MTHFD1's role in mitochondrial and cytosolic one-carbon pathways. Researchers employ this antibody to explore metabolic dysregulation in cancer, where MTHFD1 overexpression supports rapid cell proliferation, or in developmental disorders linked to folate deficiency. Its application extends to assessing metabolic adaptations in response to nutrient stress, drug targeting, or genetic models (e.g., MTHFD1 knockout studies). Proper controls, such as siRNA-mediated knockdown, are recommended to confirm signal specificity.
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