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Rabbit Polyclonal MTHFD1(CenterP550) Antibody

  • 中文名: MTHFD1 (Center P550)抗体
  • 别    名: C-1-tetrahydrofolate synthase, cytoplasmic, C1-THF synthase, Methylenetetrahydrofolate dehydrogenase, Methenyltetrahydrofolate cyclohydrolase, Formyltetrahydrofolate synthetase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, MTHFD1, M
货号: IPDX33642
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 1/10-1/50 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesC-1-tetrahydrofolate synthase, cytoplasmic, C1-THF synthase, Methylenetetrahydrofolate dehydrogenase, Methenyltetrahydrofolate cyclohydrolase, Formyltetrahydrofolate synthetase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, MTHFD1, MTHFC, MTHFD
Entrez GeneID4522
WB Predicted band size101.5kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis MTHFD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 535-562 amino acids from the Central region of human MTHFD1.
FormulationPurified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) .

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参考文献

以下是关于MTHFD1(Center P550)抗体的3篇参考文献示例:

1. **文献名称**:*MTHFD1 regulates mitochondrial folate metabolism and colorectal cancer progression*

**作者**:Smith A, et al.

**摘要**:本研究利用针对MTHFD1中心区域(P550表位)的特异性抗体,通过免疫印迹和免疫组化分析结直肠癌组织中MTHFD1的表达水平,发现其高表达与线粒体叶酸代谢异常及肿瘤侵袭性相关。

2. **文献名称**:*Genetic and functional analysis of MTHFD1 in neural tube defects*

**作者**:Chen L, et al.

**摘要**:通过Western blot和免疫荧光技术,使用抗MTHFD1(P550)抗体检测小鼠胚胎神经管组织中MTHFD1的亚细胞定位,揭示其叶酸代谢功能缺陷与神经管畸形的关联。

3. **文献名称**:*Targeting MTHFD1 in cardiovascular disease: Insights from a mouse model*

**作者**:Wang Y, et al.

**摘要**:研究采用针对MTHFD1中心区域的抗体(P550表位)分析动脉粥样硬化小鼠模型的血管组织,证实MTHFD1表达下调导致同型半胱氨酸累积,促进血管内皮损伤。

(注:以上文献信息为模拟示例,实际引用需根据具体文献调整。)

背景信息

The MTHFD1 (Center P550) antibody detects a central region of methylenetetrahydrofolate dehydrogenase, cyclohydrolase, and formyltetrahydrofolate synthetase 1 (MTHFD1), a bifunctional enzyme critical in one-carbon metabolism. MTHFD1 facilitates the interconversion of folate derivatives to generate precursors for nucleotide synthesis (purines, thymidylate) and methyl group donors (e.g., S-adenosylmethionine). The enzyme contains three functional domains: a dehydrogenase, cyclohydrolase, and synthetase. The "Center P550" epitope likely resides within the synthetase domain, which is involved in formyltetrahydrofolate production.

This rabbit polyclonal antibody, raised against a peptide near position P550. is commonly used in Western blotting (WB), immunohistochemistry (IHC), and immunocytochemistry (ICC) to study MTHFD1 expression and localization. It is validated for specificity in human and mouse tissues, often showing cytoplasmic staining, consistent with MTHFD1's role in mitochondrial and cytosolic one-carbon pathways. Researchers employ this antibody to explore metabolic dysregulation in cancer, where MTHFD1 overexpression supports rapid cell proliferation, or in developmental disorders linked to folate deficiency. Its application extends to assessing metabolic adaptations in response to nutrient stress, drug targeting, or genetic models (e.g., MTHFD1 knockout studies). Proper controls, such as siRNA-mediated knockdown, are recommended to confirm signal specificity.

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