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Rabbit Polyclonal Zebrafishwnt8b Antibody

  • 中文名: Zebrafish wnt8b抗体
  • 别    名: Protein Wnt-8b, wnt8b, wnt-8b
货号: IPDX33263
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/2000 zebrafish
IF 咨询技术 zebrafish
IHC 咨询技术 zebrafish
ICC 技术咨询 zebrafish
FCM 咨询技术 zebrafish
Elisa 咨询技术 zebrafish

产品详情

AliasesProtein Wnt-8b, wnt8b, wnt-8b
Entrez GeneID30144
WB Predicted band size40.3kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivityzebrafish
ImmunogenThis Zebrafish wnt8b antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 201-235 amino acids from the central region of Zebrafish wnt8b.

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参考文献

以下是关于斑马鱼Wnt8b抗体的3篇文献示例(注:内容为模拟生成,实际文献需通过学术数据库检索确认):

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1. **文献名称**: "Characterization of a zebrafish Wnt8b polyclonal antibody for embryonic studies"

**作者**: Kimura Y, et al.

**摘要**: 研究团队通过重组斑马鱼Wnt8b蛋白片段免疫兔,制备多克隆抗体。抗体经Western blot验证特异性,并在斑马鱼胚胎中检测到Wnt8b在早期神经板和中后脑区域的动态表达,支持其在神经发育中的功能研究。

2. **文献名称**: "Wnt8b regulates axial patterning via antibody-validated signaling in zebrafish"

**作者**: Hsieh JC, et al.

**摘要**: 开发了一种抗斑马鱼Wnt8b的单克隆抗体,通过免疫沉淀和免疫荧光证实其结合活性。研究利用该抗体揭示Wnt8b在胚胎体轴形成中通过调控β-catenin信号通路发挥作用,突变体胚胎中信号异常。

3. **文献名称**: "Antibody-based spatial mapping of Wnt8b expression in zebrafish gastrulation"

**作者**: Shimizu T, et al.

**摘要**: 该文献报道了一种高特异性抗体的应用,通过整胚免疫染色技术系统绘制了Wnt8b在斑马鱼原肠胚期的表达图谱,发现其在边缘区细胞中富集,提示其参与中胚层诱导和细胞迁移调控。

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如需实际文献,建议在 **PubMed** 或 **Google Scholar** 中检索关键词:`zebrafish wnt8b antibody`,筛选涉及抗体开发或应用的实验性研究。部分研究可能结合CRISPR突变体进行抗体功能验证。

背景信息

The zebrafish Wnt8b antibody is a crucial tool for studying the Wnt signaling pathway in developmental biology. Wnt8b, a member of the Wnt family of secreted glycoproteins, plays essential roles in embryonic patterning, cell fate determination, and tissue morphogenesis. In zebrafish, Wnt8b is particularly implicated in posterior brain development, neural crest cell specification, and somite formation. Researchers use anti-Wnt8b antibodies to detect the spatial and temporal expression patterns of Wnt8b protein during embryogenesis, often through techniques like Western blotting, immunohistochemistry (IHC), and whole-mount immunofluorescence. These antibodies are typically generated by immunizing hosts with purified Wnt8b peptides or recombinant proteins, yielding polyclonal or monoclonal variants. Validation often includes knockout controls to confirm specificity, as cross-reactivity with other Wnt family members (e.g., Wnt8a) can occur due to structural similarities. Commercially available or custom-produced zebrafish Wnt8b antibodies have facilitated studies on Wnt/β-catenin signaling dynamics, genetic interaction screens, and disease models involving developmental defects. Their application extends to investigating tissue regeneration, cancer biology, and neurodevelopmental disorders, leveraging zebrafish as a transparent vertebrate model. Proper antibody characterization remains critical, as Wnt proteins are highly conserved and post-translationally modified, requiring optimized protocols for reliable detection. This reagent continues to support mechanistic insights into Wnt8b's dual roles as a morphogen and signaling regulator in vivo.

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