Rabbit Polyclonal Zebrafishwnt8b Antibody

The zebrafish Wnt8b antibody is a crucial tool for studying the Wnt signaling pathway in developmental biology. Wnt8b, a member of the Wnt family of secreted glycoproteins, plays essential roles in embryonic patterning, cell fate determination, and tissue morphogenesis. In zebrafish, Wnt8b is particularly implicated in posterior brain development, neural crest cell specification, and somite formation. Researchers use anti-Wnt8b antibodies to detect the spatial and temporal expression patterns of Wnt8b protein during embryogenesis, often through techniques like Western blotting, immunohistochemistry (IHC), and whole-mount immunofluorescence. These antibodies are typically generated by immunizing hosts with purified Wnt8b peptides or recombinant proteins, yielding polyclonal or monoclonal variants. Validation often includes knockout controls to confirm specificity, as cross-reactivity with other Wnt family members (e.g., Wnt8a) can occur due to structural similarities. Commercially available or custom-produced zebrafish Wnt8b antibodies have facilitated studies on Wnt/β-catenin signaling dynamics, genetic interaction screens, and disease models involving developmental defects. Their application extends to investigating tissue regeneration, cancer biology, and neurodevelopmental disorders, leveraging zebrafish as a transparent vertebrate model. Proper antibody characterization remains critical, as Wnt proteins are highly conserved and post-translationally modified, requiring optimized protocols for reliable detection. This reagent continues to support mechanistic insights into Wnt8b's dual roles as a morphogen and signaling regulator in vivo.