Rabbit Polyclonal GLD2(N-term) Antibody

+ +

•  

   
     Western blot analysis of lysates from HepG2 cell line and mouse brain tissue lysate (from left to right), using GLD2 Antibody  (N-term)(Cat.  #P32744).  P32744 was diluted at 1:1000 at each lane.  A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.    

•  

   
     Western blot analysis of lysates from mouse brain, mouse cerebellum tissue lysate (from left to right),  using GLD2 Antibody  (N-term)(Cat.  #P32744).  P32744 was diluted at 1:1000 at each lane.  A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.    

•  

   
     Fluorescent image of MCF-7 cells stained with GLD2 Antibody (N-term)(Cat#P32744). P32744 was diluted at 1:25 dilution.  An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).  Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).    

•  

   
     Western blot analysis of lysates from mouse brain, mouse cerebellum tissue lysate (from left to right),  using GLD2 Antibody  (N-term)(Cat.  #P32744).  P32744 was diluted at 1:1000 at each lane.  A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.    

•  

   
     Immunohistochemical analysis of paraffin-embedded H. cerebellum section using GLD2 Antibody (N-term)(Cat#P32744). P32744 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.    

•  

   
     GLD2 Antibody (N-term) (Cat. #P32744) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.  This data demonstrates the use of the GLD2 Antibody (N-term) for immunohistochemistry.  Clinical relevance has not been evaluated.    

The GLD2 (N-term) antibody is a valuable tool for studying the GLD-2 (Germ Line Development-2) protein, also known as PAPD4 or TUT2. which belongs to the non-canonical poly(A) polymerase family. GLD-2 plays a critical role in post-transcriptional gene regulation by extending the poly(A) tails of specific mRNAs, enhancing their stability, translation, and cytoplasmic polyadenylation. This activity is essential in germline development, neuronal synaptic plasticity, and stem cell maintenance. The antibody specifically targets the N-terminal region of GLD-2. enabling researchers to investigate its expression, localization, and interactions in various biological contexts.

GLD-2 functions as a cytoplasmic RNA-binding protein, often partnering with RNA-binding proteins like CPEB (Cytoplasmic Polyadenylation Element Binding protein) to regulate mRNA metabolism. Its involvement in cell reprogramming, oocyte maturation, and neuronal signaling underscores its importance in developmental and cellular processes. The GLD2 (N-term) antibody is widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to explore these mechanisms. Its specificity for the N-terminal domain allows differentiation from other poly(A) polymerases, aiding in studies of tissue-specific expression patterns and functional redundancy. Research utilizing this antibody has advanced understanding of mRNA dynamics in gametogenesis, neurobiology, and cancer, where dysregulated polyadenylation contributes to disease progression.