纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | UBAP1 |
Uniprot No | Q9NZ09 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-502aa |
氨基酸序列 | MASKKLGADF HGTFSYLDDV PFKTGDKFKT PAKVGLPIGF SLPDCLQVVR EVQYDFSLEK KTIEWAEEIK KIEEAEREAE CKIAEAEAKV NSKSGPEGDS KMSFSKTHST ATMPPPINPI LASLQHNSIL TPTRVSSSAT KQKVLSPPHI KADFNLADFE CEEDPFDNLE LKTIDEKEEL RNILVGTTGP IMAQLLDNNL PRGGSGSVLQ DEEVLASLER ATLDFKPLHK PNGFITLPQL GNCEKMSLSS KVSLPPIPAV SNIKSLSFPK LDSDDSNQKT AKLASTFHST SCLRNGTFQN SLKPSTQSSA SELNGHHTLG LSALNLDSGT EMPALTSSQM PSLSVLSVCT EESSPPNTGP TVTPPNFSVS QVPNMPSCPQ AYSELQMLSP SERQCVETVV NMGYSYECVL RAMKKKGENI EQILDYLFAH GQLCEKGFDP LLVEEALEMH QCSEEKMMEF LQLMSKFKEM GFELKDIKEV LLLHNNDQDN ALEDLMARAG AS |
预测分子量 | kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBAP1重组蛋白的3篇示例参考文献(部分为虚构示例,实际文献需通过学术数据库验证):
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1. **文献名称**:*UBAP1 is a component of the ESCRT-I complex essential for MVB sorting*
**作者**:Stefani F, et al.
**摘要**:研究通过重组UBAP1蛋白表达和免疫共沉淀实验,证实UBAP1是ESCRT-I复合体的关键亚基,参与多泡体(MVB)的货物分选,并调控泛素化依赖的膜蛋白降解。
2. **文献名称**:*Structural basis of ubiquitin recognition by the UBAP1 subunit of ESCRT-I*
**作者**:Zhang Y, et al.
**摘要**:利用重组UBAP1蛋白进行结构生物学分析,揭示了其N端泛素结合结构域(UBD)的晶体结构,阐明其通过结合泛素化信号调控内体膜重塑的分子机制。
3. **文献名称**:*UBAP1 regulates autophagosome biogenesis by stabilizing the ESCRT-I complex*
**作者**:Tanaka K, et al.
**摘要**:通过体外重组UBAP1与ESCRT-I复合体的功能重建实验,发现UBAP1通过维持复合体稳定性促进自噬小体形成,缺失UBAP1导致自噬功能异常。
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**注意**:以上文献为示例,实际研究中请通过PubMed、Web of Science等平台以关键词“UBAP1 recombinant”或“UBAP1 ESCRT”检索最新文献。真实研究可能包括对UBAP1重组蛋白的功能验证、结构解析或其在疾病模型中的作用分析。
UBAP1 (Ubiquitin-associated protein 1) is a multifunctional protein encoded by the UBAP1 gene, primarily recognized for its role in the endosomal sorting complex required for transport (ESCRT) pathway. As a component of the ESCRT-I complex, UBAP1 facilitates the sorting of ubiquitinated membrane proteins into intraluminal vesicles (ILVs) of multivesicular bodies (MVBs), a critical step in lysosomal degradation and extracellular vesicle biogenesis. Structurally, it contains a ubiquitin-binding UBA domain and a C-terminal STAM-binding region, enabling interactions with ubiquitinated cargoes and other ESCRT subunits. Its function in membrane remodeling and protein trafficking is essential for maintaining cellular homeostasis, autophagy, and receptor downregulation.
UBAP1 has gained attention due to its involvement in neurodegenerative diseases and cancer. Mutations or dysregulation of UBAP1 are linked to hereditary spastic paraplegia (HSP) and amyotrophic lateral sclerosis (ALS), likely through impaired protein quality control. In oncology, UBAP1 overexpression correlates with tumor progression in cancers like hepatocellular carcinoma and glioblastoma, where it may promote invasion and drug resistance by modulating ESCRT-dependent pathways. Additionally, UBAP1 interacts with viral proteins, influencing viral budding in infections like HIV-1.
Recombinant UBAP1 proteins are engineered using expression systems (e.g., E. coli, mammalian cells) for functional studies. These proteins enable in vitro analysis of ESCRT assembly, ubiquitin-dependent cargo recognition, and structural characterization. Researchers also utilize UBAP1 variants to dissect disease mechanisms or screen for therapeutic compounds targeting ESCRT dysfunction. As an emerging biomarker and therapeutic target, UBAP1 continues to inspire studies exploring its molecular interactions and pathophysiological roles.
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