关键信息
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基因名
repD
- 应用
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别名
repD; Replication initiation protein
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种属
Staphylococcus aureus
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表达系统
E. coli
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标签
N- His-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P03065
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表达区间
1-311aa
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分子量
53.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
RepD recombinant protein is a significant focus in molecular biology and biotechnology due to its crucial role in DNA replication, particularly in plasmid maintenance and mobilization in bacterial systems. The RepD protein, derived from various plasmids, functions as a replication initiator, facilitating the unwinding of DNA and subsequent synthesis, which is essential for the propagation of plasmids within bacterial populations. Understanding the biochemistry and functionality of RepD is vital for applications such as genetic engineering, synthetic biology, and developing biotechnological tools. Researchers are particularly interested in characterizing its structural properties, interaction with other proteins, and regulatory mechanisms that govern its activity. Furthermore, the manipulation of RepD or similar proteins can lead to advancements in plasmid design for gene therapy, vaccine development, and novel antimicrobial strategies. By harnessing the properties of RepD, scientists aim to create more efficient systems for gene delivery and expression in various host organisms, contributing to improved outcomes in both research and therapeutic contexts. The ongoing studies on RepD recombinant protein not only enhance the understanding of DNA replication but also open new avenues for innovative biotechnological applications.
