| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HCFC2 |
| Uniprot No | Q9Y5Z7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-792aa |
| 氨基酸序列 | MAAPSLLNWR RVSSFTGPVP RARHGHRAVA IRELMIIFGG GNEGIADELH VYNTATNQWF LPAVRGDIPP GCAAHGFVCD GTRILVFGGM VEYGRYSNEL YELQASRWLW KKVKPHPPPS GLPPCPRLGH SFSLYGNKCY LFGGLANESE DSNNNVPRYL NDFYELELQH GSGVVGWSIP VTKGVVPSPR ESHTAVIYCK KDSGSPKMYV FGGMCGARLD DLWQLDLETM SWSKPETKGT VPLPRSLHTA SVIGNKMYIF GGWVPHKGEN TETSPHDCEW RCTSSFSYLN LDTTEWTTLV SDSQEDKKNS RPRPRAGHCA VAIGTRLYFW SGRDGYKKAL NSQVCCKDLW YLDTEKPPAP SQVQLIKATT NSFHVKWDEV STVEGYLLQL STDLPYQAAS SDSSAAPNMQ GVRMDPHRQG SNNIVPNSIN DTINSTKTEQ PATKETSMKN KPDFKALTDS NAILYPSLAS NASNHNSHVV DMLRKNEGPH TSANVGVLSS CLDVRTVIPE TSVSSTVSST QTMVTQQTIK TESSSTNGAV VKDETSLTTF STKSEVDETY ALPATKISRV ETHATATPFS KETPSNPVAT VKAGERQWCD VGIFKNNTAL VSQFYLLPKG KQSISKVGNA DVPDYSLLKK QDLVPGTGYR FRVAAINGCG IGPFSKISEF KTCIPGFPGA PSAVRISKNV EGIHLSWEPP TSPSGNILEY SAYLAIRTAQ IQDNPSQLVF MRIYCGLKTS CIVTAGQLAN AHIDYTSRPA IVFRISAKNE KGYGPATQVR WLQGNNKKAP LN |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HCFC2重组蛋白的假设性参考文献示例(实际文献需通过学术数据库查询)：
1. **文献名称**：Structural and Functional Analysis of HCFC2 Recombinant Protein in Transcriptional Regulation
**作者**：Smith J, et al.
**摘要**：研究利用大肠杆菌表达系统纯化HCFC2重组蛋白，通过X射线晶体学解析其三维结构，并发现其通过与RNA聚合酶II亚基相互作用参与基因转录调控。
2. **文献名称**：HCFC2 Recombinant Protein Interaction with Viral Promoters: Implications for Host-Pathogen Interactions
**作者**：Lee H, et al.
**摘要**：通过Co-IP和荧光素酶报告基因实验，证明HCFC2重组蛋白能特异性结合疱疹病毒早期启动子区域，调控病毒基因表达，提示其在抗病毒免疫中的潜在作用。
3. **文献名称**：HCFC2 Knockdown and Recombinant Rescue in Neural Development Models
**作者**：Garcia R, et al.
**摘要**：在神经干细胞中敲低HCFC2导致分化异常，而外源添加纯化的HCFC2重组蛋白可部分恢复神经元突触形成，表明其在神经发育中的关键功能。
4. **文献名称**：High-Yield Production of HCFC2 Recombinant Protein Using Mammalian Expression Systems
**作者**：Wang Y, et al.
**摘要**：优化哺乳动物HEK293细胞表达体系，实现HCFC2重组蛋白的高效分泌表达，并通过质谱验证其翻译后修饰对蛋白稳定性的影响。
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注：以上为模拟示例，实际文献需通过PubMed/Google Scholar检索关键词(如"HCFC2 recombinant protein"、"Host Cell Factor C2")，建议结合具体研究方向筛选。
**Background of HCFC2 Recombinant Protein**
HCFC2 (Host Cell Factor C2) is a member of the HCF protein family, which plays critical roles in transcriptional regulation and cell-cycle progression. Originally identified as a component of the human herpes simplex virus (HSV) host-cell factor complex, HCFC2 shares structural and functional similarities with HCF1. a well-studied transcriptional co-regulator. The protein is characterized by multiple Kelch repeat domains, which mediate protein-protein interactions, and a conserved HCF N-terminal domain involved in chromatin remodeling and gene expression modulation.
HCFC2 is implicated in diverse cellular processes, including cell proliferation, differentiation, and epigenetic regulation. It interacts with chromatin-modifying enzymes (e.g., histone acetyltransferases) and transcription factors, influencing pathways such as Wnt/β-catenin and nuclear factor-κB (NF-κB). Dysregulation of HCFC2 has been linked to developmental disorders, cancer, and neurodegenerative diseases, highlighting its biological and clinical relevance.
Recombinant HCFC2 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains functional domains essential for biochemical and cellular studies. It serves as a tool to investigate HCFC2's molecular interactions, post-translational modifications (e.g., proteolytic processing), and role in viral latency-reactivation cycles (e.g., HSV). Additionally, recombinant HCFC2 aids in drug discovery by enabling high-throughput screening for inhibitors or stabilizers targeting its activity in disease contexts.
Despite progress, challenges remain in fully elucidating HCFC2's pleiotropic functions, partly due to its complex domain architecture and context-dependent interactions. Ongoing research aims to clarify its tissue-specific roles and therapeutic potential, leveraging recombinant protein technology to advance mechanistic and translational studies.
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