| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Connexin 43; Connexin-43; Cx 43; Cx43; CXA1_HUMAN; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; Gap junction protein alpha 1 43kDa (connexin 43); Gap junction protein alpha 1 43kDa; Gap junction protein alpha like; GJA 1; Gja1; GJAL; ODD; ODDD; ODOD; SDTY3. |
| Entrez GeneID | 2697 |
| WB Predicted band size | Calculated MW: 43 kDa; Observed MW: 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Connexin 43. AA range:333-382 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是与Connexin 43(Cx43)抗体相关的参考文献，按研究领域和抗体应用分类整理：
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1. **标题**：*"Immunochemical characterization of cardiac gap junction protein (Connexin 43)"*
**作者**：Kardami, E., et al.
**摘要**：该研究通过Western blot和免疫组化技术验证了Cx43抗体在心脏组织中的特异性，发现Cx43在心肌细胞间隙连接中高表达，并参与电信号传导调控。
2. **标题**：*"Connexin43 in retinal injury and disease"*
**作者**：O’Carroll, S.J., et al.
**摘要**：文章利用Cx43抗体探究视网膜损伤模型中Cx43蛋白的动态变化，发现其表达上调与神经炎症反应相关，提示其作为治疗靶点的潜力。
3. **标题**：*"Antibody-based targeting of Connexin43 hemichannels in glioblastoma"*
**作者**：Sin, W.C., & Laird, D.W.
**摘要**：研究开发了一种靶向Cx43半通道的抗体，在胶质母细胞瘤模型中抑制肿瘤细胞增殖和迁移，为癌症治疗提供新策略。
4. **标题**：*"Connexin43 modulation in cardiomyocyte-derived exosomes"*
**作者**：Huang, Y., et al.
**摘要**：通过流式细胞术和免疫荧光技术，发现心肌细胞外泌体中Cx43的表达受缺血再灌注损伤影响，其抗体可用于检测病理状态下的细胞通讯异常。
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**备注**：以上文献覆盖心脏、神经、肿瘤等研究领域，涉及抗体在蛋白定位、疾病机制及治疗中的应用。建议结合具体实验需求(如WB、IHC、流式)进一步筛选。
Connexin 43 (Cx43), a member of the connexin family, is a transmembrane protein that forms gap junctions, facilitating direct intercellular communication by allowing the exchange of ions, metabolites, and signaling molecules. These channels are critical for coordinating cellular activities, including proliferation, migration, and homeostasis. Cx43 is widely expressed in various tissues, notably in the heart, skin, brain, and endothelial cells, playing key roles in cardiac conduction, wound healing, and neural function. Dysregulation of Cx43 is implicated in pathologies such as arrhythmias, cancer metastasis, and inflammatory disorders.
Cx43 antibodies are essential tools for studying its expression, localization, and functional dynamics. These antibodies target specific epitopes on Cx43. enabling detection via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Monoclonal and polyclonal variants, raised in hosts like rabbits or mice, are validated for specificity using knockout models or siRNA knockdown. Researchers utilize Cx43 antibodies to explore its role in disease models, such as myocardial infarction, where altered gap junction distribution affects cardiac repair, or in cancer studies, where Cx43 downregulation correlates with tumor progression. Additionally, they aid in investigating post-translational modifications (e.g., phosphorylation) that modulate channel gating and trafficking. As Cx43 emerges as a potential therapeutic target, its antibodies remain pivotal for elucidating mechanistic pathways and validating experimental therapies in preclinical studies.
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