| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PYCARD; ASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; hASC; Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1 |
| Entrez GeneID | 29108 |
| WB Predicted band size | Calculated MW: 22 kDa; Observed MW: 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ASC. AA range:10-59 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于ASC抗体的3-4篇代表性文献的简要总结(基于模拟数据，仅供参考)：
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1. **文献名称**：*The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-β*
**作者**：Martinon, F., et al.
**摘要**：该研究首次提出ASC蛋白作为炎症小体(inflammasome)的核心组分，通过CARD结构域介导caspase-1的激活，促进IL-1β的成熟。文中利用ASC抗体验证其在免疫细胞中的表达及与炎症小体其他组分的相互作用，为后续研究提供了关键工具。
2. **文献名称**：*ASC speck formation as a readout of inflammasome activation*
**作者**：Sutterwala, F. Y., et al.
**摘要**：本文开发了一种基于ASC抗体的免疫荧光检测方法，证实炎症小体激活后ASC会聚集成“斑点”(speck)。研究通过ASC抗体标记，在巨噬细胞和体内模型中可视化炎症小体动态，为炎症性疾病机制研究提供直观方法。
3. **文献名称**：*Autoantibodies against ASC in autoimmune diseases: diagnostic and functional implications*
**作者**：Shimizu, K., et al.
**摘要**：研究发现系统性红斑狼疮(SLE)患者血清中存在抗ASC自身抗体，通过ELISA和Western blot结合ASC抗体检测，揭示其与疾病活动度的相关性，并探讨了这些抗体可能干扰炎症小体功能的机制。
4. **文献名称**：*Targeting ASC in tumor-associated macrophages enhances antitumor immunity*
**作者**：Li, X., et al.
**摘要**：利用ASC特异性抗体阻断肿瘤微环境中巨噬细胞的炎症小体活化，发现可减少免疫抑制性细胞因子分泌，增强T细胞抗肿瘤反应。研究为癌症免疫治疗提供了新靶点。
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**注**：以上文献为示例性质，实际引用需根据具体研究方向检索PubMed、Web of Science等数据库获取最新及权威论文。
ASC (Apoptosis-associated speck-like protein containing a CARD) is a critical adaptor protein in the innate immune system, primarily involved in inflammasome assembly and regulation. It contains two functional domains: an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD). These domains mediate protein-protein interactions, enabling ASC to bridge sensor proteins (e.g., NLRs, AIM2) and downstream effector caspases (e.g., caspase-1) during inflammasome activation. This process drives the cleavage and secretion of pro-inflammatory cytokines (IL-1β, IL-18) and induces pyroptosis, a form of inflammatory cell death.
ASC antibodies are essential tools for studying inflammasome-related pathways in diseases like autoimmune disorders, infections, and cancer. They detect ASC expression, oligomerization ("ASC speck" formation), and subcellular localization via techniques like Western blot, immunofluorescence, and immunohistochemistry. Dysregulated ASC activity is linked to chronic inflammation, making it a biomarker for conditions such as rheumatoid arthritis, Alzheimer's disease, and sepsis. Additionally, ASC-deficient mouse models, validated using these antibodies, highlight its role in pathogen clearance and homeostasis. Research on ASC antibodies also explores therapeutic targeting of inflammasomes to modulate immune responses. Their specificity and reliability are crucial for elucidating mechanisms of inflammation-driven pathologies and developing precision therapies.
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