| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CCND1; BCL1; D11S287E; PRAD1; U21B31; Cyclin D1 |
| Entrez GeneID | 595 |
| WB Predicted band size | Calculated MW: 34 kDa; Observed MW: 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Cyclin D1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Cyclin D1抗体的3篇参考文献示例(文献信息为模拟示例，实际引用需核实)：
1. **"Cyclin D1 as a therapeutic target in cancer"**
*作者：Motokura T, Arnold A*
摘要：该研究探讨Cyclin D1在肿瘤发生中的作用，通过抗体检测其在多种癌症中的过表达，提示其可作为潜在治疗靶点。
2. **"Immunohistochemical detection of Cyclin D1 in paraffin-embedded tissue sections for diagnostic use"**
*作者：Hall PA, Richards MA*
摘要：研究验证了Cyclin D1抗体在福尔马林固定组织中的特异性，提出其作为套细胞淋巴瘤等癌症的可靠诊断标志物。
3. **"Cyclin D1 expression in breast cancer: Correlation with prognosis and hormone receptor status"**
*作者：Gillett C, Smith P*
摘要：通过Cyclin D1抗体分析乳腺癌样本，发现其高表达与ER阳性肿瘤及不良预后相关，支持其在临床分型中的应用。
注：以上为简化示例，实际文献需查阅PubMed等数据库获取准确信息。
Cyclin D1. a key regulator of the cell cycle, belongs to the cyclin protein family and plays a critical role in driving the G1-to-S phase transition by forming complexes with cyclin-dependent kinases (CDKs), particularly CDK4 and CDK6. Its expression is tightly controlled by extracellular mitogenic signals, and dysregulation of Cyclin D1 is strongly linked to cancer pathogenesis. Overexpression of Cyclin D1. often due to gene amplification or chromosomal translocations (e.g., in mantle cell lymphoma), promotes uncontrolled cell proliferation and tumorigenesis. As a result, Cyclin D1 serves as both a diagnostic biomarker and a potential therapeutic target in various cancers.
Cyclin D1 antibodies are essential tools in research and diagnostics for detecting Cyclin D1 protein expression. These antibodies are widely used in techniques such as immunohistochemistry (IHC), Western blotting, and immunofluorescence (IF) to assess Cyclin D1 levels in tissue samples or cell lines. In clinical settings, Cyclin D1 IHC is particularly valuable for diagnosing mantle cell lymphoma, where nuclear staining helps differentiate it from other B-cell neoplasms. Researchers also utilize these antibodies to study cell cycle mechanisms, drug responses, and oncogenic signaling pathways.
When using Cyclin D1 antibodies, specificity and validation are crucial due to potential cross-reactivity with other cyclins or splice variants. Proper controls and standardized protocols ensure reliable results, aiding in accurate disease classification and advancing targeted cancer therapies.
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