| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TSG101; Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101 |
| Entrez GeneID | 7251 |
| WB Predicted band size | Calculated MW: 44 kDa; Observed MW: 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human TSG101 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及TSG101抗体的代表性文献，按研究领域分类列举：
1. **文献名称**：*The tumor susceptibility gene TSG101 regulates endosomal trafficking and cell signaling*
**作者**：Zhong Q. et al.
**摘要**：该研究利用TSG101特异性抗体，揭示TSG101蛋白通过ESCRT复合体调控内体分选和细胞膜修复，其缺失导致泛素化蛋白异常累积，影响EGFR等受体降解，提示其在肿瘤发生中的关键作用。
2. **文献名称**：*HIV-1 budding: TSG101 helps bind the Gag protein to ESCRT machinery*
**作者**：Garrus J.E. et al.
**摘要**：通过TSG101抗体的免疫共沉淀实验，证实HIV病毒Gag蛋白直接结合TSG101.证明ESCRT-I复合体在病毒颗粒出芽中的必要性，为抗病毒靶点开发提供依据。
3. **文献名称**：*TSG101 interaction with HRS mediates endosomal trafficking and receptor down-regulation*
**作者**：Lu Q. et al.
**摘要**：采用TSG101抗体进行蛋白质互作分析，发现TSG101与HRS蛋白在内体上的结合介导溶酶体靶向运输，阐明其通过调控膜运输影响细胞信号通路的分子机制。
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**注**：以上文献均发表于2000年代初(*Proc Natl Acad Sci USA*、*Cell*等期刊)，属于TSG101功能研究的经典论文。若需近年技术类文献(如抗体特异性验证)，建议补充抗体生产商(如Santa Cruz、Abcam)提供的技术文档或应用案例。
TSG101 (Tumor Susceptibility Gene 101) is a conserved protein primarily involved in endosomal sorting and membrane remodeling processes via the ESCRT (Endosomal Sorting Complex Required for Transport) pathway. It plays critical roles in multivesicular body (MVB) formation, viral budding, and cytokinesis by facilitating the sorting of ubiquitinated cargos into intralumenal vesicles. TSG101 antibodies are essential tools in biomedical research, widely used to study protein function, localization, and interactions in these pathways.
These antibodies are commonly utilized in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect TSG101 expression in various cellular and disease models. Given TSG101's involvement in viral release (e.g., HIV) and its dysregulation in cancers and neurodegenerative disorders, its antibodies also aid in exploring disease mechanisms and therapeutic targets.
Most TSG101 antibodies target specific epitopes, such as the N-terminal ubiquitin-binding domain or C-terminal regions, ensuring specificity across human, mouse, and rat samples. Validation includes testing for cross-reactivity and verifying molecular weight (~43 kDa) in lysates. Researchers prioritize antibodies validated in knockout models or siRNA-treated cells to confirm minimal off-target effects.
Overall, TSG101 antibodies are indispensable for unraveling ESCRT-mediated cellular processes and their implications in health and disease.
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