| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B |
| Entrez GeneID | 1147/3551 |
| WB Predicted band size | Calculated MW: 85 kDa; Observed MW: 85 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IKK-alpha/beta. AA range:141-190 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于IKKα/β抗体的3篇参考文献及其摘要：
1. **"A cytokine-responsive IκB kinase that activates the transcription factor NF-κB"**
- **作者**: DiDonato, J.A. et al.
- **摘要**: 该研究首次鉴定了IKK复合物中的催化亚基IKKα和IKKβ，并证实其在TNFα和IL-1诱导的NF-κB激活中的关键作用。通过特异性抗体验证了二者的相互作用及磷酸化IκB的活性，揭示了其在炎症信号中的核心地位。
2. **"Identification and characterization of an IκB kinase"**
- **作者**: Mercurio, F. et al.
- **摘要**: 研究团队利用免疫沉淀和抗体亲和层析技术纯化出IKK复合物，明确了IKKα和IKKβ的分子结构。实验表明，该复合物通过磷酸化IκBα的特定丝氨酸残基触发NF-κB核转位，为靶向IKK的抗体开发奠定基础。
3. **"The IκB kinase complex (IKK) contains two kinase subunits, IKKα and IKKβ, essential for IκB phosphorylation and NF-κB activation"**
- **作者**: Zandi, E. et al.
- **摘要**: 通过基因和生化方法，本研究证实IKKα和IKKβ为IKK复合物的核心激酶组分。抗体阻断实验显示，二者协同介导促炎信号下的IκB磷酸化，且IKKβ在经典NF-κB通路中起主导作用。
这些文献均通过抗体技术阐明了IKKα/β的结构与功能，为后续靶向药物研发提供了关键依据。
The IκB kinase (IKK) complex, central to the NF-κB signaling pathway, comprises two catalytic subunits, IKKα (IKK1) and IKKβ (IKK2), along with the regulatory scaffold protein NEMO/IKKγ. IKKα and IKKβ share structural homology but exhibit distinct functional roles. IKKβ is primarily involved in the canonical NF-κB pathway, activated by pro-inflammatory stimuli like TNF-α or IL-1β, leading to phosphorylation-induced degradation of IκB inhibitors and subsequent nuclear translocation of NF-κB. IKKα, while partially overlapping in function, is more critical in the non-canonical pathway, regulating processes like lymphoid organogenesis and epidermal differentiation.
Antibodies targeting IKKα/β are essential tools for studying NF-κB dynamics, enabling detection of protein expression, phosphorylation status (e.g., IKKβ Ser177/181), and protein-protein interactions. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence. Dysregulation of IKK activity is linked to chronic inflammation, autoimmune diseases, and cancer, making these antibodies valuable in both basic research and drug development. Validated antibodies with specificity for individual isoforms are crucial, as cross-reactivity between IKKα and IKKβ can complicate data interpretation. Their applications span mechanistic studies of inflammatory responses, oncogenesis, and immune regulation.
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