| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110 |
| Entrez GeneID | 5925 |
| WB Predicted band size | Calculated MW: 106 kDa; Observed MW: 106 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Retinoblastoma. AA range:751-800 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于Rb抗体的参考文献示例(内容为虚构，仅供格式参考)：
1. **"Characterization of Rb-specific antibodies for cell cycle studies"**
*作者：Smith et al. (2018)*
摘要：本研究比较了多种商品化Rb抗体的特异性与灵敏度，通过Western blot和免疫荧光验证其在细胞周期调控研究中的适用性，推荐Clone X7C6适用于磷酸化Rb蛋白检测。
2. **"Role of Rb phosphorylation in cancer progression: Insights from antibody-based assays"**
*作者：Lee & Chen (2015)*
摘要：利用Rb磷酸化特异性抗体(pRb-Ser807/811)分析肿瘤样本，揭示Rb失活与乳腺癌转移的相关性，强调抗体选择对临床诊断的重要性。
3. **"A novel monoclonal antibody targeting the N-terminal domain of Retinoblastoma protein"**
*作者：Garcia et al. (2020)*
摘要：开发了一种新型Rb单克隆抗体(mAb-RbN1)，验证其在小鼠模型和人类组织中的高亲和力，适用于染色质免疫共沉淀(ChIP)研究Rb与E2F的相互作用。
4. **"Optimization of Rb antibody staining protocols for formalin-fixed tissues"**
*作者：Patel et al. (2016)*
摘要：系统优化了福尔马林固定石蜡包埋(FFPE)组织中Rb抗原修复条件，提升抗体(Clone 3C8)的染色一致性，为病理学提供标准化方案。
(注：以上文献为示例，实际引用请检索PubMed等数据库获取真实研究。)
**Background of Rb Antibody**
The retinoblastoma (Rb) protein, encoded by the *RB1* gene, is a critical tumor suppressor regulating cell cycle progression. Discovered through studies on retinoblastoma, a childhood eye cancer, Rb’s role in inhibiting uncontrolled cell division established it as a prototype tumor suppressor. Functionally, Rb binds to E2F transcription factors, blocking cell cycle entry from G1 to S phase. Its activity is modulated by phosphorylation; hypophosphorylated Rb actively suppresses E2F, while cyclin-dependent kinase (CDK)-mediated phosphorylation inactivates Rb, enabling cell cycle progression.
Mutations or deletions in *RB1* disrupt this regulation, leading to uncontrolled proliferation and tumorigenesis. Beyond retinoblastoma, Rb pathway alterations are implicated in diverse cancers, including osteosarcoma and lung cancer.
Rb antibodies are essential tools in research, enabling detection and analysis of Rb expression, phosphorylation status, and interactions via techniques like Western blot, immunohistochemistry, and immunofluorescence. These antibodies help elucidate Rb’s role in cancer biology, cell cycle control, and therapeutic responses. Additionally, they aid in diagnosing Rb-deficient tumors and evaluating targeted therapies, such as CDK inhibitors, underscoring their importance in both basic and clinical oncology research.
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