| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | HIST1H2BB; H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f; HIST1H2BC; H2BFL; HIST1H2BE; H2BFH; HIST1H2BF; H2BFG; HIST1H2BG; H2BFA; HIST1H2BI; H2BFK; Histone H2B type 1-C/E/F/G/I; Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l; HIST1H2BD; H2BFB; HIRIP2; Histone H2B type 1-D; HIRA-interacting protein 2; Histone H2B.1 B; Histone H2B.b; H2B/b; HIST1H2BH; H2BFJ; Histone H2B type 1-H; Histone H2B.j; H2B/j; HIST1H2BJ; H2BFR; Histone H2B type 1-J; Histone H2B.1; Histone H2B.r; H2B/r; HIST1H2BK; H2BFT; HIRIP1; Histone H2B type 1-K; H2B K; HIRA-interacting protein 1; HIST1H2BL; H2BFC; Histone H2B type 1-L; Histone H2B.c; H2B/c; HIST1H2BM; H2BFE; Histone H2B type 1-M; Histone H2B.e; H2B/e; HIST1H2BN; H2BFD; Histone H2B type 1-N; Histone H2B.d; H2B/d; HIST1H2BO; H2BFH; H2BFN; Histone H2B type 1-O; Histone H2B.2; Histone H2B.n; H2B/n |
| Entrez GeneID | 3017/8339/8343/8344/8346/8347/3018/8345/85236/8340/8342/8341/440689 |
| WB Predicted band size | Calculated MW: 14 kDa; Observed MW: 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from the Internal region of human Histone H2B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Histone H2B抗体的3篇参考文献，按文献名称、作者和摘要内容概括整理：
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1. **文献名称**: *"A Monoclonal Antibody Against Histone H2B Specifically Recognizes a Phosphorylated Epitope in Mitotic Cells"*
**作者**: Kimura H, et al.
**摘要**: 该研究开发了一种特异性识别组蛋白H2B磷酸化形式(Ser33位点)的单克隆抗体，并证明其在有丝分裂细胞中的特异性标记作用，可用于细胞周期动态研究。
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2. **文献名称**: *"Histone H2B Ubiquitination: A Key Regulator of Chromatin Dynamics and DNA Repair"*
**作者**: Weake VM, et al.
**摘要**: 文献综述了组蛋白H2B泛素化修饰在染色质重塑和DNA修复中的作用，并强调特异性抗体在检测H2B泛素化状态中的关键应用，为表观遗传调控机制提供工具支持。
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3. **文献名称**: *"Autoantibodies to Histone H2B in Systemic Lupus Erythematosus: Correlation with Disease Activity"*
**作者**: van Bavel CC, et al.
**摘要**: 研究发现系统性红斑狼疮(SLE)患者血清中存在抗组蛋白H2B的自身抗体，其水平与疾病活动性相关，提示H2B抗体可作为潜在的生物标志物用于临床评估。
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如需具体文献来源(期刊卷号、页码等)，建议通过PubMed或Google Scholar输入标题或作者信息进一步检索。
Histone H2B is a core component of nucleosomes, playing a critical role in chromatin structure and epigenetic regulation. As one of the five major histone families (H1. H2A, H2B, H3. H4), H2B forms a dimer with H2A, contributing to the nucleosome octamer around which DNA is wrapped. This structural organization regulates DNA accessibility for transcription, replication, and repair processes. Post-translational modifications (PTMs) of H2B, such as ubiquitination, phosphorylation, and acetylation, are dynamically regulated and influence chromatin remodeling, gene expression, and cellular responses to stress.
Antibodies targeting Histone H2B are essential tools for studying its localization, modifications, and interactions. They are widely used in techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and flow cytometry. Specific H2B antibodies can distinguish between unmodified and modified forms (e.g., H2B ubiquitination in DNA repair or apoptosis) or detect cell type-specific variants. Dysregulation of H2B or its PTMs has been implicated in cancer, neurodegenerative diseases, and autoimmune disorders. For example, autoantibodies against H2B are observed in systemic lupus erythematosus (SLE), highlighting its clinical relevance. Researchers also utilize H2B antibodies to investigate replication-coupled chromatin assembly, histone exchange dynamics, and crosstalk with other epigenetic marks. As chromatin biology advances, H2B antibodies remain vital for dissecting its multifaceted roles in health and disease.
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