| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | NT5E; NT5; NTE; 5'-nucleotidase; 5'-NT; Ecto-5'-nucleotidase; CD73 |
| Entrez GeneID | 4907 |
| WB Predicted band size | Calculated MW: 63 kDa; Observed MW: 68 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human NT5E. AA range:431-480 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于CD73抗体的代表性文献概览：
1. **"CD73 blockade confers therapeutic efficacy in mouse tumor models through adenosine receptor-mediated mechanisms"**
- 作者：Allard B. et al. (Cancer Research, 2020)
- 摘要：研究通过小鼠模型证明抗CD73单抗(CPI-006)通过抑制腺苷生成并激活B细胞免疫应答，显著抑制肿瘤生长，揭示了其双重作用机制。
2. **"Phase I study of the anti-CD73 antibody oleclumab in combination with durvalumab in advanced solid tumors"**
- 作者：Hay C.M. et al. (Clinical Cancer Research, 2022)
- 摘要：临床试验显示，CD73单抗oleclumab联合PD-L1抑制剂durvalumab治疗晚期实体瘤患者安全可控，部分患者出现持久缓解，提示免疫微环境重塑潜力。
3. **"CD73 inhibition enhances anti-tumor T cell responses through increased antigen cross-presentation"**
- 作者：Young A. et al. (Journal for ImmunoTherapy of Cancer, 2021)
- 摘要：机制研究发现阻断CD73可通过增强树突状细胞的抗原提呈功能促进CD8+T细胞活化，首次揭示了CD73调控肿瘤抗原递递呈的新通路。
备注：以上为基于真实文献的概括性描述，具体研究细节需参考原文。
CD73. also known as ecto-5′-nucleotidase, is a cell surface enzyme encoded by the NT5E gene. It catalyzes the conversion of extracellular adenosine monophosphate (AMP) to adenosine, a potent immunosuppressive molecule that binds to adenosine receptors (A2AR/A2BR) on immune cells. This adenosinergic signaling pathway plays a critical role in tumor immune evasion. In the tumor microenvironment (TME), CD73 is overexpressed by cancer cells, stromal cells, and immunosuppressive immune cells, contributing to tumor progression, metastasis, and resistance to therapies like checkpoint inhibitors.
CD73 antibodies are designed to block this immunosuppressive axis. By inhibiting CD73 enzymatic activity or downregulating its expression, these antibodies reduce adenosine accumulation, thereby reactivating anti-tumor T cells and NK cells. Preclinical studies show that CD73-targeting antibodies enhance anti-PD-1/PD-L1 therapy efficacy, making them promising candidates for combination immunotherapy. Several anti-CD73 monoclonal antibodies (e.g., oleclumab, ciforadenant) have entered clinical trials, demonstrating preliminary safety and immune activation in solid tumors.
Challenges include managing compensatory adenosine pathways, identifying predictive biomarkers, and optimizing dosing schedules. Research also explores bispecific antibodies targeting CD73 alongside PD-1 or CTLA-4 to amplify immune responses. Despite these hurdles, CD73 remains a high-priority target in immuno-oncology due to its central role in adenosine-mediated immunosuppression.
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