| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | POLR3G; DNA-directed RNA polymerase III subunit RPC7; RNA polymerase III subunit C7; DNA-directed RNA polymerase III subunit G; RNA polymerase III 32 kDa subunit; RPC32 |
| Entrez GeneID | 10622 |
| WB Predicted band size | Calculated MW: 26 kDa; Observed MW: 26 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human POLR3G. AA range:151-200 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于RNA Polymerase III Subunit C7抗体的部分参考文献示例(注：文献为模拟概括，实际需查询数据库获取具体信息)：
1. **文献名称**：*Autoantibodies against RNA Polymerase III Subunit C7 in Systemic Sclerosis*
**作者**：Smith A, et al.
**摘要**：研究报道了系统性硬化症(硬皮病)患者血清中针对RNA Pol III C7亚基的自身抗体的高特异性，揭示其与疾病进展和器官纤维化的相关性。
2. **文献名称**：*Structural Insights into RNA Polymerase III Assembly via RPC7 Interaction*
**作者**：Chen L, et al.
**摘要**：通过免疫共沉淀和冷冻电镜技术，利用C7抗体解析RNA Pol III复合体的组装机制，阐明RPC7在酶活性调控中的作用。
3. **文献名称**：*POLR3C Mutations and Antibody Validation in Neurodevelopmental Disorders*
**作者**：Wang Y, et al.
**摘要**：验证了针对POLR3C(C7亚基)的抗体的特异性，并发现该亚基突变可能导致小头畸形等神经发育异常，为基因诊断提供工具。
4. **文献名称**：*RNA Polymerase III Dysregulation in Cancer: Role of C7-Specific Antibodies*
**作者**：Garcia R, et al.
**摘要**：开发高亲和力C7抗体用于检测多种癌症中RNA Pol III的异常表达，提示其作为肿瘤生物标志物的潜力。
建议通过PubMed或Google Scholar搜索关键词“RNA Pol III C7 antibody”或“POLR3C antibody”获取真实文献。
The RNA Polymerase III (Pol III) Subunit C7 antibody targets the C7 subunit, a critical component of the RNA Pol III enzyme complex responsible for transcribing small non-coding RNAs, including tRNAs, 5S rRNA, and other regulatory RNAs. The C7 subunit, encoded by the *POLR3G* (or *RPC7α*) and *POLR3GL* (or *RPC7β*) genes in humans, exists as two isoforms with distinct expression patterns. While POLR3G is primarily expressed in undifferentiated or proliferating cells, POLR3GL is ubiquitously present across tissues, suggesting isoform-specific regulatory roles in development and cellular homeostasis.
Antibodies against the C7 subunit are widely used to study Pol III assembly, transcriptional regulation, and its dysregulation in diseases. These antibodies enable detection and localization of the subunit via techniques like Western blotting, immunoprecipitation, and immunofluorescence. Researchers employ them to explore Pol III's interaction with transcription factors (e.g., TFIIIB) and its responsiveness to cellular signals, such as mTOR pathway activation. Additionally, C7 antibodies contribute to investigations linking Pol III dysfunction to cancers, neurodegenerative disorders, and developmental syndromes like hypomyelinating leukodystrophy.
As a molecular tool, the RNA Pol III Subunit C7 antibody supports mechanistic studies of transcriptional control, ribosome biogenesis, and stress responses, while also holding potential as a diagnostic marker for Pol III-related pathologies.
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