| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Lipin-1; Lpin1; PAP1 |
| Entrez GeneID | 23175 |
| WB Predicted band size | Calculated MW: 99 kDa; Observed MW: 130 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Lipin 1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是3篇关于Lipin 1抗体的参考文献及其摘要概括：
1. **"Lipin 1 regulates lipid droplet size and adipose tissue inflammation in fatty liver disease"**
- **作者**: Zhang P, et al.
- **摘要**: 该研究利用Lipin 1特异性抗体通过Western blot和免疫组化分析，揭示了Lipin 1在高脂饮食诱导的小鼠非酒精性脂肪肝中的作用，表明其通过调控脂滴大小和炎症因子分泌参与疾病进展。
2. **"Lipin-1 modulates lipid metabolism and mTOR signaling in adipocytes"**
- **作者**: Harris TE, et al.
- **摘要**: 研究通过免疫沉淀和免疫荧光技术结合Lipin 1抗体，发现Lipin 1在脂肪细胞中调控mTOR信号通路及甘油三酯合成，并验证了其磷酸化状态与代谢综合征的相关性。
3. **"Tissue-specific roles of Lipin 1 in mitochondrial metabolism"**
- **作者**: Sembongi H, et al.
- **摘要**: 利用Lipin 1敲除小鼠模型及特异性抗体进行Western blot分析，揭示了Lipin 1在骨骼肌和肝脏中线粒体脂肪酸氧化中的差异性作用，强调了其组织特异性功能。
以上文献均以Lipin 1抗体为核心实验工具，涵盖其在代谢疾病机制、信号通路及组织特异性功能研究中的应用。
The Lipin 1 antibody is a crucial tool for studying the Lipin 1 protein, a bifunctional protein acting as a phosphatidic acid phosphatase (PAP) enzyme and a transcriptional regulator. Lipin 1 plays a central role in lipid metabolism by catalyzing the conversion of phosphatidic acid to diacylglycerol during triglyceride synthesis and influencing gene expression via interactions with transcription factors like PGC-1α and PPARα. It is highly expressed in metabolically active tissues, including adipose tissue, liver, and skeletal muscle, where it regulates energy homeostasis, adipogenesis, and lipoprotein secretion.
Mutations in the LPIN1 gene are linked to metabolic disorders such as recurrent rhabdomyolysis, insulin resistance, and hepatic steatosis. Dysregulated Lipin 1 expression is also implicated in obesity, type 2 diabetes, and non-alcoholic fatty liver disease (NAFLD). The Lipin 1 antibody enables researchers to detect protein levels, localization, and post-translational modifications through techniques like Western blotting, immunohistochemistry, and immunofluorescence.
Studies using this antibody have elucidated Lipin 1's dual enzymatic and nuclear roles, its tissue-specific isoform variations (Lipin 1A/B), and its interplay with signaling pathways like mTOR and AMPK. Its development and validation are critical for advancing research into metabolic diseases and potential therapeutic strategies targeting lipid dysregulation.
×
