| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | WDR5; BIG3; WD repeat-containing protein 5; BMP2-induced 3-kb gene protein |
| Entrez GeneID | 11091 |
| WB Predicted band size | Calculated MW: 37 kDa; Observed MW: 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human WDR5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于WDR5抗体的3篇参考文献及其摘要(文献为虚拟示例，仅供格式参考)：
1. **标题**：WDR5 regulates chromatin recruitment and enzymatic activity of the MLL1 histone methyltransferase complex
**作者**：Smith J, Patel R, Andrews F
**摘要**：该研究利用WDR5抗体通过ChIP-seq和免疫共沉淀技术，揭示了WDR5在招募MLL复合体至HOX基因启动子区域的关键作用，并证明其缺失会显著降低H3K4甲基化水平，影响白血病细胞的增殖。
2. **标题**：Targeting WDR5 as a therapeutic strategy in MYC-driven cancers
**作者**：Dey A, Wong E, Wei Y
**摘要**：研究通过Western blot和免疫荧光技术(使用WDR5特异性抗体)，发现WDR5与c-Myc蛋白在多种癌症中形成功能协同，抑制WDR5可阻断肿瘤生长。抗体在验证WDR5敲低效果及定位分析中发挥关键作用。
3. **标题**：Structural basis of WDR5 interactions with RbBP5 and histone H3 in histone modification complexes
**作者**：Liu X, Zhang Q, Allis CD
**摘要**：该研究通过免疫沉淀结合质谱分析(依赖WDR5抗体)，解析了WDR5与RbBP5及组蛋白H3的结合界面，为设计靶向WDR5的小分子抑制剂提供了结构基础，并验证了抗体在蛋白质相互作用研究中的特异性。
4. **标题**：WDR5 mediates neuroblastoma progression via maintaining stemness gene expression
**作者**：Thomas M, Kim S, Jones L
**摘要**：利用WDR5抗体进行ChIP-qPCR和流式细胞分选，研究发现WDR5在神经母细胞瘤中通过维持SOX2和NANOG等干性基因的表达促进肿瘤恶性转化，提示其作为潜在治疗靶点。
(注：以上文献信息为模拟生成，实际引用请核实真实数据库。)
WDR5 (WD Repeat Domain 5) is a conserved nuclear protein belonging to the WD40-repeat protein family, known for its scaffolding role in multi-subunit complexes regulating epigenetic processes. It is a core component of the MLL/COMPASS (Complex of Proteins Associated with Set1) histone methyltransferase complexes, which catalyze histone H3 lysine 4 methylation (H3K4me), a hallmark of transcriptionally active chromatin. WDR5 mediates interactions between MLL complexes and histone substrates or other regulatory proteins, influencing gene expression, cell differentiation, and proliferation. Dysregulation of WDR5 is linked to cancers, developmental disorders, and metabolic diseases, making it a target for therapeutic exploration.
WDR5 antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like immunoprecipitation, chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to investigate its role in chromatin remodeling, transcriptional regulation, and disease mechanisms. Researchers also utilize these antibodies to assess WDR5’s involvement in cancer progression, particularly in leukemias and solid tumors driven by MLL rearrangements or overexpression. Commercial WDR5 antibodies are typically raised against epitopes in its N-terminal or WD40-repeat domains, with validation emphasizing specificity across human and model organisms. Challenges include distinguishing WDR5 from paralogs and ensuring minimal cross-reactivity in multiplex assays.
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