| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BAP1; DING; BAP-1; HIPI3; RING2; RING1B |
| Entrez GeneID | 6045 |
| WB Predicted band size | Calculated MW: 38 kDa; Observed MW: 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human RING2 / RING1B / RNF2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是3-4篇关于RING2抗体的示例文献(内容为虚构示例，仅供参考)：
1. **文献名称**：RING2 regulates H2A ubiquitination and gene silencing in mammalian cells
**作者**：Wang et al., 2016
**摘要**：本研究利用RING2特异性抗体，通过ChIP-seq和Western blot揭示了RING2在PRC1复合物中催化组蛋白H2A泛素化的关键作用，并证明其与基因沉默的相关性。
2. **文献名称**：Development of a monoclonal antibody targeting RING2 for cancer biomarker studies
**作者**：Smith et al., 2018
**摘要**：报道了一种新型RING2单克隆抗体的开发，验证了其在免疫组化中的特异性，并发现RING2在多种实体瘤中异常高表达，提示其作为癌症诊断标志物的潜力。
3. **文献名称**：RING2 interacts with BMI1 to maintain stem cell pluripotency
**作者**：Li et al., 2020
**摘要**：通过免疫共沉淀(Co-IP)和RING2抗体的应用，阐明了RING2与BMI1蛋白的相互作用机制及其在维持胚胎干细胞多能性中的功能。
4. **文献名称**：RING2 deficiency leads to embryonic lethality in mice
**作者**：Garcia et al., 2015
**摘要**：利用RING2敲除小鼠模型和特异性抗体检测，证明RING2缺失导致胚胎发育异常，强调了其在早期发育中的必要性。
---
注：以上为模拟文献，真实文献需通过PubMed/Google Scholar等平台检索关键词(如"RING2 antibody" "RNF2" "Polycomb")。
RING2 antibody targets the RING2 protein, a critical component of the Polycomb Repressive Complex 1 (PRC1), which plays a central role in epigenetic gene silencing. RING2. also known as RNF2. functions as an E3 ubiquitin ligase responsible for catalyzing the monoubiquitination of histone H2A at lysine 119 (H2AK119ub), a hallmark of transcriptionally repressive chromatin. This modification facilitates chromatin compaction and suppresses the expression of genes involved in differentiation, cell cycle regulation, and stem cell maintenance. PRC1. in coordination with PRC2 (which methylates histone H3K27), maintains cellular identity by silencing developmental regulators during embryogenesis and tissue homeostasis.
RING2 antibodies are widely used in research to investigate PRC1-mediated epigenetic mechanisms, including chromatin remodeling, X-chromosome inactivation, and genomic imprinting. They enable techniques like chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to study RING2's localization, protein interactions (e.g., with BMI1 or PHC proteins), and dysregulation in diseases. Aberrant RING2 expression is linked to cancers, where its overexpression may drive oncogenesis by repressing tumor suppressor genes. Additionally, RING2 antibodies aid in exploring therapeutic strategies targeting Polycomb complexes in cancer and developmental disorders. These tools are essential for understanding how PRC1 dysfunction contributes to pathological states and stem cell pluripotency.
×
