首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人前列腺特异性抗原前体(Pro-PSA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Pro-PSA含量。Pro-PSA即前列腺特异性抗原前体，在前列腺癌的诊断和病情评估中有重要意义。其研究机制主要是通过检测体内Pro-PSA含量，与总PSA等指标结合，能更准确地鉴别前列腺癌与良性前列腺疾病，提高早期诊断的准确性。试剂盒检测范围为125 pg/mL-4000 pg/mL，该产品适用于探索前列腺疾病分子机制、评估生物标志物表达特征、筛选潜在治疗靶点等基础研究，也可用于细胞模型或动物模型中Pro - PSA的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Pro-PSA
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-4000 pg/mL
灵敏度：62.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<20% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-PSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 84-94 | |||||
| 1:4 | Average % | 105 | ||||
| Range % | 98-108 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of human Pro-PSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 83-94 | ||||
| EDTA plasma (n=4) | 93 | 89-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average40002.3512.2012.27620001.4121.3561.38410000.8480.8260.8372500.2340.2470.2411250.1050.1090.107 | ||||||
货期：3-5 working days
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