首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人纤维蛋白原α链(FGA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGA含量。FGA（纤维蛋白原α链）在凝血、炎症等生理病理过程中发挥重要作用。研究发现它可影响凝血级联反应，促进血栓形成。其结构与功能的关系、与疾病发生发展机制等是研究热点，有望为血栓性疾病等的诊治提供新靶点和思路。试剂盒检测范围为0.94 μg/mL-60 μg/mL，适用于科研领域对凝血功能调控机制、遗传性出血性疾病模型构建、抗血栓药物作用靶点探索等研究，亦可为心血管疾病或肝脏合成功能相关的动物实验提供凝血分子层面的数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FGA ELISA Kit; FGB ELISA Kit; FGG ELISA Kit; Fib2 ELISA Kit; FIBA_HUMAN ELISA Kit; Fibrinogen A alpha polypeptide ELISA Kit; Fibrinogen alpha chain ELISA Kit; Fibrinogen B alpha polypeptide ELISA Kit; Fibrinogen beta chain ELISA Kit; Fibrinogen G alpha polypeptide ELISA Kit; Fibrinogen gamma chain ELISA Kit; fibrinogen; B beta polypeptide ELISA Kit; fibrinogen; G gamma polypeptide ELISA Kit; fibrinogen; gamma polypeptide ELISA Kit; Fibrinogen--alpha -polypeptide chain ELISA Kit; Fibrinogen--beta -polypeptide chain ELISA Kit; Fibrinogen--gamma-polypeptide chain ELISA Kit
缩写：FGA
Uniprot No.：P02671
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.94 μg/mL-60 μg/mL
灵敏度：0.47 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FGA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:200 | Average % | 101 | ||||
| Range % | 95-105 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human FGA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 97 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 600.0800.0820.081 300.1830.1760.180 150.3990.4040.402 7.50.6760.6390.658 3.751.2561.2241.240 1.881.5541.5901.572 0.941.7851.8291.807 02.1492.0972.123 | ||||||
货期：3-5 working days
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