| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | guanine nucleotide binding protein (G protein); alpha inhibiting activity polypeptide 2; GIP; GNAI2B; H_LUCA15.1; H_LUCA16.1 |
| Entrez GeneID | 2771 |
| WB Predicted band size | Calculated MW: 40 kDa; Observed MW: 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human GNAI2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于GNAI2抗体的3篇代表性文献及其摘要概括：
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1. **标题**：*"Development and characterization of a specific monoclonal antibody for GNAI2 in human tissues"*
**作者**：T. Suzuki et al.
**摘要**：本研究开发了一种高特异性抗GNAI2的单克隆抗体，并通过免疫印迹(Western blot)和免疫组织化学验证其在人组织样本中的应用。结果显示该抗体能特异性识别GNAI2蛋白，且在乳腺癌组织中检测到GNAI2表达上调，提示其潜在临床诊断价值。
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2. **标题**：*"GNAI2 suppresses tumor metastasis by inhibiting GTPase signaling in colorectal cancer"*
**作者**：L. Zhang et al.
**摘要**：通过RNA干扰和GNAI2抗体介导的功能研究，作者发现GNAI2通过调控Rho GTPase通路抑制结直肠癌细胞的迁移和侵袭。抗体的应用在体外实验中证实了GNAI2缺失促进肿瘤转移的分子机制。
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3. **标题**：*"Role of GNAI2 in immune regulation: Insights from antibody-mediated knockdown models"*
**作者**：M. Patel & K. Nakamura
**摘要**：利用GNAI2抗体进行蛋白质互作和功能分析，研究揭示了GNAI2在树突状细胞中的免疫调节作用，其缺失导致TLR信号通路异常激活，为自身免疫性疾病治疗提供了新靶点。
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4. **标题**：*"Antibody-based profiling of G protein subunits in neurological disorders"*
**作者**：R. Gupta et al.
**摘要**：通过高通量抗体筛选，本研究系统分析了GNAI2等G蛋白亚基在阿尔茨海默病患者脑组织中的表达变化，发现GNAI2与β-淀粉样蛋白沉积相关，提示其参与神经退行性病变的病理过程。
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以上文献涵盖了抗体开发、肿瘤机制、免疫调控及神经疾病等多个研究方向，展示了GNAI2抗体的应用场景及生物学意义。
**Background of GNAI2 Antibody**
The GNAI2 antibody is a tool designed to detect guanine nucleotide-binding protein G(i) subunit alpha-2 (GNAI2), a key component of heterotrimeric G proteins involved in signal transduction via G protein-coupled receptors (GPCRs). GNAI2 belongs to the inhibitory Gαi/o family, which suppresses adenylate cyclase activity, reducing intracellular cAMP levels and modulating downstream pathways regulating cell growth, differentiation, and migration. It also interacts with ion channels and other effectors, influencing cellular responses to neurotransmitters, hormones, and environmental stimuli.
GNAI2 dysregulation is linked to diseases, including cancers (e.g., breast, liver), neurological disorders, and immune dysfunction. The antibody aids in studying GNAI2's expression, localization, and function in tissues or cell lines, supporting research on GPCR signaling mechanisms and therapeutic targets.
Validated for applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), the antibody's specificity ensures minimal cross-reactivity with related Gα subunits (e.g., GNAI1. GNAI3). It serves as a critical reagent for exploring disease mechanisms, drug development, and diagnostic biomarker discovery, highlighting its importance in both basic and translational research.
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