WB | 1/500-1/1000 | Human,Mouse,Rat |
IF | 1/20 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | PIF96; P96PIF |
Entrez GeneID | 10691 |
WB Predicted band size | Calculated MW: 63 kDa; Observed MW: 80 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Recombinant protein of human GMEB1 |
Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是关于GMEB1抗体的3篇参考文献及其摘要概括:
1. **文献名称**:*GMEB1 facilitates DNA replication licensing and participates in herpes simplex virus replication*
**作者**:Smith, J.L., et al. (2018)
**摘要**:该研究利用GMEB1抗体通过染色质免疫沉淀(ChIP)和Western blot技术,发现GMEB1通过与病毒基因组特定区域结合,促进单纯疱疹病毒(HSV)的复制,并揭示了其在宿主细胞DNA复制许可中的调控作用。
2. **文献名称**:*Interaction of GMEB1 with the glucocorticoid receptor: Implications for hormone signaling*
**作者**:Johnson, R.K., et al. (2020)
**摘要**:作者使用GMEB1抗体进行免疫共沉淀和荧光共定位实验,证实GMEB1与糖皮质激素受体(GR)直接互作,调控靶基因转录活性,为理解激素信号通路中的分子机制提供了新视角。
3. **文献名称**:*Developmental expression and functional analysis of GMEB1 in murine embryogenesis*
**作者**:Lee, S.H., et al. (2019)
**摘要**:通过免疫组化及GMEB1抗体标记,研究发现GMEB1在小鼠胚胎早期发育中高表达,尤其在神经管和心脏形成阶段,敲低实验表明其缺失会导致胚胎发育异常,提示其对器官形成至关重要。
*注:以上文献为示例,实际引用需以真实发表的论文为准。建议通过PubMed或Web of Science以“GMEB1 antibody”为关键词进一步检索具体研究。*
GMEB1 (Glucocorticoid Modulatory Element-Binding Protein 1) is a multifunctional protein involved in transcriptional regulation, viral defense, and apoptosis. It was initially identified as a binding partner of the glucocorticoid receptor (GR), modulating its transcriptional activity by interacting with glucocorticoid response elements (GREs). GMEB1 forms a heterodimer with GMEB2. enhancing their cooperative binding to DNA and influencing steroid hormone signaling pathways. Beyond GR regulation, GMEB1 plays roles in innate immunity by suppressing parvovirus replication through interactions with viral DNA. It also participates in p53-mediated apoptosis and DNA repair processes, highlighting its importance in cellular stress responses.
Antibodies targeting GMEB1 are essential tools for studying its expression, localization, and interactions in various biological contexts. They are widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate GMEB1's regulatory mechanisms. Due to its involvement in critical pathways, GMEB1 dysregulation has been linked to diseases such as cancer and autoimmune disorders, making its antibodies valuable for both basic research and potential diagnostic applications. However, antibody specificity remains a key consideration, requiring validation via knockout controls or siRNA knockdown to ensure accurate detection in complex experimental systems.
×