纯度 | >95%SDS-PAGE. |
种属 | Human |
靶点 | FIS1 |
Uniprot No | Q9Y3D6 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-122aa |
氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MEAVLNELVS VEDLLKFEKK FQSEKAAGSV SKSTQFEYAW CLVRSKYNDD IRKGIVLLEE LLPKGSKEEQ RDYVFYLAVG NYRLKEYEKA LKYVRGLLQT EPQNNQAKEL ERLIDKAMKK DG |
预测分子量 | 16 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FIS1重组蛋白的3篇参考文献,按文献名称、作者和摘要内容简要概括:
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1. **文献名称**:*Mdm12 and Mmm1/Fis1 act as a molecular tether between mitochondria and the plasma membrane*
**作者**:Koch A, et al.
**摘要**:该研究揭示了酵母中Fis1蛋白与Mdm12、Mmm1的相互作用,通过重组蛋白实验证明Fis1在介导线粒体与细胞膜之间的物理连接中起关键作用,调控线粒体形态和分布。
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2. **文献名称**:*Structural insights into the interaction of human Fis1 with the mitochondrial fission protein Mff*
**作者**:Zhang Y, et al.
**摘要**:通过重组人源Fis1蛋白的体外表达和结构分析,阐明了Fis1与线粒体分裂因子Mff的结合机制,揭示了Fis1的TPR结构域在招募下游分裂蛋白Drp1中的关键作用。
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3. **文献名称**:*Fis1 regulates mitochondrial dynamics through inhibition of the fusion machinery*
**作者**:Yu R, et al.
**摘要**:研究利用重组Fis1蛋白进行功能实验,发现其不仅参与线粒体分裂,还通过抑制融合蛋白MFN1/2的活性维持线粒体动态平衡,为病理状态下线粒体功能障碍提供机制解释。
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以上文献涵盖FIS1在结构、分子互作及功能调控方面的研究,均为领域内代表性工作。如需扩展或特定方向文献,可进一步补充。
**Background of FIS1 Recombinant Protein**
FIS1 (Fission Mitochondrial 1) is a conserved eukaryotic protein critical for regulating mitochondrial and peroxisomal fission, processes essential for maintaining cellular organelle dynamics. Initially identified in yeast, FIS1 homologs have been characterized in mammals, plants, and other organisms. Structurally, FIS1 contains a C-terminal transmembrane domain anchoring it to the outer mitochondrial or peroxisomal membrane and an N-terminal tetratricopeptide repeat (TPR) domain that facilitates interactions with downstream fission machinery components, such as dynamin-related proteins (e.g., DRP1).
Recombinant FIS1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cell cultures) to enable in vitro studies of its structure, function, and interactions. The recombinant form is typically purified via affinity tags (e.g., His-tag) and validated for activity in binding assays or fission reconstitution experiments. Its production has advanced research into mitochondrial dynamics, particularly in understanding how dysregulated fission contributes to pathologies like neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), metabolic disorders, and cancer.
Studies using FIS1 recombinant protein have elucidated its role in apoptosis, mitophagy, and cellular stress responses. For instance, excessive mitochondrial fission mediated by FIS1/DRP1 is linked to fragmented mitochondrial networks in cancer cells, influencing metastasis and chemoresistance. Conversely, impaired FIS1 function correlates with aggregated mitochondria and neuronal degeneration.
Beyond basic research, recombinant FIS1 serves as a tool for drug screening targeting mitochondrial dysfunction. Inhibitors or enhancers of FIS1-mediated fission hold therapeutic potential, driving interest in structural and functional characterization. Despite progress, questions remain about post-translational modifications, tissue-specific regulation, and interplay with fusion proteins (e.g., MFN1/2). Continued use of recombinant FIS1 is vital for unraveling these complexities in organelle biology and disease mechanisms.
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