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产品描述：綿羊5羟色胺/血清素/血清胺(5HT/ST)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的5HT/ST含量。5HT即5-羟色胺，是人体内重要神经递质。5HT/ST靶点相关研究聚焦其在调节情绪、认知等方面作用机制。通过药物作用于该靶点，可调节5HT水平与功能，用于治疗如抑郁症、焦虑症等精神疾病，目前仍在不断深入探索以挖掘更多临床价值。试剂盒检测范围为0.625 ng/mL-10 ng/mL，适用于绵羊模型在神经生物学、药理学及动物行为学研究中的应用，例如评估应激状态下神经递质动态变化、药物干预对血清素系统的影响或消化道功能相关机制探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：5HT/ST
种属：Ovis aries (Sheep)
样本类型：serum, plasma
检测范围：0.625 ng/mL-10 ng/mL
灵敏度：0.312 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Biochemicals
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep 5HT/ST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:40 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:80 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:160 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of sheep 5HT/ST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 100.1220.1150.119 50.2340.2160.225 2.50.4040.4070.406 1.250.7430.7390.741 0.6251.2211.1941.208 02.0511.9592.005 | ||||||
货期：3-5 working days
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