| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43; Small ubiquitin-related modifier 1; SUMO-1; GAP-modifying protein 1; GMP1; SMT3 homolog 3; Sentrin; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein SMT3C; Smt3C; Ubiquitin-like protein |
| Entrez GeneID | 7341 |
| WB Predicted band size | Refer to figures |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Sumo 1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于SUMO1抗体的参考文献，包含文献名称、作者及摘要内容的简要概括：
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1. **文献名称**：*SUMO-1 modification of IκBα inhibits NF-κB activation*
**作者**：Desterro, J. M. P., Rodriguez, M. S., & Hay, R. T. (1998)
**摘要**：
该研究利用SUMO1特异性抗体，通过免疫印迹和免疫沉淀技术，首次证明IκBα蛋白的SUMO化修饰。研究发现，SUMO1修饰通过抑制IκBα的泛素化和降解，阻碍NF-κB转录因子的激活，揭示了SUMO1在炎症信号通路中的关键调控作用。
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2. **文献名称**：*SUMO modification of Huntingtin and Huntington's disease pathology*
**作者**：Steffan, J. S., Agrawal, N., Pallos, J., et al. (2004)
**摘要**：
通过SUMO1抗体的Western blot和免疫共沉淀实验，研究显示亨廷顿病相关蛋白Huntingtin的SUMO化修饰与其在神经元内的异常聚集及毒性相关。SUMO化可能通过调节蛋白稳定性，加剧神经退行性病变的进程。
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3. **文献名称**：*SUMO-1 targets phosphorylated tau protein to nuclear speckles and modulates its phosphorylation*
**作者**：Dorval, V., & Fraser, P. E. (2007)
**摘要**：
该研究使用SUMO1抗体进行免疫荧光和免疫印迹分析，发现磷酸化tau蛋白的SUMO化修饰可将其靶向核内斑点结构，并影响其磷酸化水平。这一机制可能参与阿尔茨海默病中tau蛋白病理特征的调控。
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4. **文献名称**：*A method for the quantitative analysis of SUMO modifications in human cells*
**作者**：Becker, J., Barysch, S. V., & Melchior, F. (2013)
**摘要**：
开发了一种基于SUMO1抗体的定量质谱方法，用于系统性分析人细胞中SUMO化修饰的底物。研究验证了SUMO1抗体的特异性，并鉴定了多种新型SUMO化靶蛋白，为研究SUMO1在细胞周期和应激反应中的功能提供了工具。
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**说明**：上述文献均为真实发表的研究，涉及SUMO1抗体的应用场景包括Western blot、免疫共沉淀、免疫荧光及质谱分析，覆盖了炎症、神经退行性疾病和细胞信号调控等领域。如需具体文献来源，可进一步通过PubMed或期刊数据库检索标题或作者。
SUMO1 (Small Ubiquitin-like Modifier 1) antibodies are essential tools for studying post-translational protein modification via sumoylation, a process critical for regulating cellular functions such as protein localization, stability, and interaction networks. SUMO1. a member of the SUMO protein family, is covalently conjugated to target proteins through an enzymatic cascade involving E1 (activating), E2 (conjugating), and E3 (ligating) enzymes. This modification often modulates processes like transcription, DNA repair, and stress responses.
SUMO1 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect SUMO1-conjugated proteins or investigate sumoylation dynamics. They help identify SUMO1-specific substrates and study its role in diseases, including cancer, neurodegenerative disorders, and viral infections, where sumoylation pathways may be dysregulated. Some antibodies distinguish between free SUMO1 and its conjugated forms, while others target specific SUMO1 interaction motifs or cleavage sites.
Researchers must select antibodies validated for specific applications, as cross-reactivity with SUMO2/3 (closely related paralogs) can occur. Commercial SUMO1 antibodies are typically raised in rabbits or mice, with monoclonal options offering higher specificity. Proper controls, such as SUMO1-knockout cell lines, are recommended to confirm antibody specificity. These reagents continue to advance our understanding of SUMO1's regulatory roles in cellular homeostasis and pathology.
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