首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人裂解微管相关蛋白tau(C-MAPT/C-TAU)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、脑脊液（CSF）、组织匀浆、细胞裂解物样本中的C-MAPT/C-TAU含量。C-MAPT/C-TAU是研究tau蛋白的基因，tau蛋白是一种参与微管稳定的微管相关蛋白。tau蛋白的异常磷酸化和聚集与阿尔茨海默病等神经退行性疾病密切相关。研究C-MAPT/C-TAU有助于理解tau蛋白在神经退行性疾病中的作用机制，为开发新的治疗策略提供理论基础。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于科研领域对tau蛋白的浓度分析；可用于评估疾病模型中的tau蛋白动态变化、探究神经退行机制及药物干预效果研究，为神经病理学、分子生物学实验提供可靠工具，适用于实验室基础研究与转化医学探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：cleaved form of DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU, G protein beta1/gamma2 subunit-interacting factor 1|microtubule-associated protein tau, isoform 4
缩写：C-MAPT/C-TAU
种属：Homo sapiens (Human)
样本类型：serum, plasma, cerebrospinal fluid (CSF), tissue homogenates, cell lysates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C-MAPT/C-TAU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 102 | ||||
| Range % | 96-106 | |||||
| 1:10 | Average % | 100 | ||||
| Range % | 90-104 | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 85-99 | |||||
| 1:40 | Average % | 94 | ||||
| Range % | 88-99 | |||||
回收率：
| The recovery of human C-MAPT/C-TAU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 82-95 | ||||
| EDTA plasma (n=4) | 100 | 91-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40003.0012.8892.9452.83220002.1141.9762.0451.93210001.2311.2001.2161.1035000.5960.5650.5810.4682500.3530.3410.3470.2341250.2390.2320.2360.12362.50.1710.1680.1700.05700.1110.1150.113 | ||||||
货期：3-5 working days
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