| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 2810449N18Rik; AI043036; Mono ADP ribosyltransferase sirtuin 6; NAD-dependent protein deacetylase sirtuin-6; Regulatory protein SIR2 homolog 6; Regulatory protein SIR2 homolog; SIR2 like 6; SIR2 like protein 6; Sir2 related protein type 6; SIR2-like protein 6; SIR2 |
| Entrez GeneID | 51548 |
| WB Predicted band size | Calculated MW: 39 kDa; Observed MW: 39 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human SIRT6 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于SIRT6抗体的参考文献及其简要摘要：
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1. **"SIRT6 is a histone H3 lysine 9 deacetylase that modulates telomeric chromatin"**
- **作者**: Michishita, E. et al. (2008)
- **摘要**: 研究通过染色质免疫沉淀(ChIP)和Western blot技术，使用SIRT6特异性抗体证实其作为组蛋白H3K9去乙酰化酶的功能，并揭示SIRT6缺失导致端粒异染色质不稳定和DNA损伤增加。
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2. **"SIRT6 links histone H3 lysine 9 deacetylation to NF-κB-dependent gene expression and organismal lifespan"**
- **作者**: Kawahara, T.L. et al. (2009)
- **摘要**: 利用SIRT6抗体进行免疫荧光和Western blot分析，发现SIRT6通过抑制NF-κB通路调控衰老相关基因表达，其过表达可延长小鼠寿命。
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3. **"SIRT6 represses LINE1 retrotransposons by histone H3K9 deacetylation and heterochromatin formation"**
- **作者**: Tasselli, L. et al. (2016)
- **摘要**: 研究通过ChIP-seq和免疫沉淀结合SIRT6抗体，揭示SIRT6通过形成异染色质抑制LINE1逆转录转座子活性，维持基因组稳定性。
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4. **"SIRT6 suppresses cancer through control of Lamin A and metabolism"**
- **作者**: Klein, M.A. et al. (2011)
- **摘要**: 使用SIRT6抗体进行免疫组化分析，发现SIRT6在代谢活跃组织中高表达，并通过调控核纤层蛋白A(Lamin A)和糖代谢抑制肿瘤发生。
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以上文献均涉及SIRT6抗体的应用(如Western blot、ChIP、免疫荧光等)，并聚焦于其功能机制(如基因组稳定性、衰老、代谢调控)。
SIRT6. a member of the sirtuin family of NAD+-dependent deacylases, plays a critical role in genomic stability, DNA repair, aging, and metabolic regulation. It functions as a chromatin-associated enzyme, primarily involved in deacetylation and mono-ADP-ribosylation of histones and other substrates, influencing gene expression and stress response pathways. SIRT6's involvement in lifespan extension and its dual role in cancer (as a tumor suppressor or promoter, depending on context) have spurred significant research interest.
SIRT6 antibodies are essential tools for studying its expression, localization, and post-translational modifications in various biological contexts. These antibodies enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Commercial SIRT6 antibodies are typically validated for specificity using knockout cell lines or tissues to minimize cross-reactivity with other sirtuins. However, variability in antibody performance across studies has been noted, emphasizing the need for rigorous validation.
Research applications span cancer biology (e.g., assessing SIRT6's role in tumor metabolism), metabolic disorders (e.g., glucose homeostasis), and aging-related pathways. SIRT6 antibodies also aid in exploring its interaction partners and regulatory mechanisms, such as its modulation by NAD+ levels or oxidative stress. Ongoing studies focus on SIRT6 as a therapeutic target, driving demand for reliable antibodies to support preclinical and translational investigations.
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