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Rabbit Monoclonal SDHA Antibody

  • 中文名： SDHA抗体
  • 别    名： SDHA; SDH2; SDHF; Succinate dehydrogenase [ubiquinone] flavoprotein subunit; mitochondrial; Flavoprotein subunit of complex II; Fp
货号: IPDX21987
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesSDHA; SDH2; SDHF; Succinate dehydrogenase [ubiquinone] flavoprotein subunit; mitochondrial; Flavoprotein subunit of complex II; Fp
Entrez GeneID6389
WB Predicted band sizeCalculated MW: 73 kDa; Observed MW: 73 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthetic peptide of human SDHA
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于SDHA抗体的3篇参考文献及其摘要简述：

1. **《SDHA Immunohistochemistry Detects Germline SDHA Gene Mutations in Apparently Sporadic Paragangliomas and Pheochromocytomas》**

- 作者：Killian, J.K. 等(2011)

- 摘要：研究验证了SDHA抗体在副神经节瘤和嗜铬细胞瘤中的免疫组化应用，发现SDHA蛋白表达缺失与SDHA基因种系突变相关，为遗传性肿瘤的筛查提供依据。

2. **《Succinate Dehydrogenase (SDH)-Deficient Pancreatic Neuroendocrine Tumor: A Potential Diagnostic Pitfall》**

- 作者：Mete, O. 等(2017)

- 摘要：通过SDHA/SDHB抗体分析胰腺神经内分泌肿瘤，发现SDHA表达缺失与SDH复合体功能障碍相关，提示抗体检测在鉴别诊断中的重要性。

3. **《SDHA Loss-of-Function Mutations in KIT/PDGFRA Wild-Type Gastrointestinal Stromal Tumors》**

- 作者：Miettinen, M. 等(2013)

- 摘要：研究使用SDHA抗体检测KIT/PDGFRA野生型胃肠道间质瘤，发现SDHA蛋白缺失与患者预后不良相关，支持其作为分子分型的标志物。

这些文献展示了SDHA抗体在肿瘤遗传突变检测及诊断中的关键作用。

背景信息

The SDHA antibody is a crucial tool in biomedical research, targeting the succinate dehydrogenase complex subunit A (SDHA), a key component of mitochondrial complex II. This enzyme plays a dual role in the tricarboxylic acid (TCA) cycle and the electron transport chain, linking energy production pathways. SDHA antibodies are widely used to detect SDHA protein expression, localization, and dysfunction in various diseases. Mutations in the SDHA gene are associated with hereditary paraganglioma/pheochromocytoma, gastrointestinal stromal tumors (GISTs), and mitochondrial disorders. Researchers employ these antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study SDHA’s role in tumorigenesis, metabolic reprogramming, and oxidative stress. Additionally, SDHA is often used as a loading control in mitochondrial protein studies due to its stable expression under many conditions, though its reliability in certain pathologies (e.g., SDHA-deficient tumors) requires validation. The antibody’s specificity and sensitivity are critical for distinguishing SDHA-related deficiencies from other mitochondrial defects, aiding in diagnostics and mechanistic studies of mitochondrial dysfunction. Recent advances also explore its utility in assessing therapeutic responses targeting metabolic pathways in cancer and neurodegenerative diseases.

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