首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人抗生长激素(GH)抗体酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的GH Ab含量。人抗生长激素抗体是一种针对生长激素的特异性抗体，其研究背景涉及生长激素在人体中的作用及其相关疾病的治疗。该抗体通过阻断生长激素的作用，可用于治疗因生长激素过量引起的疾病，如肢端肥大症等。研究机制主要关注其与生长激素受体的相互作用及其在体内的药理效应。试剂盒检测范围为7.8 pg/mL-500 pg/mL，该产品适用于基础医学研究中对GH相关疾病的机制探索、药物干预效果评估或内分泌调控研究，例如在动物模型或体外实验中分析抗体与GH的相互作用，或评估特定病理状态下GH抗体表达动态变化，为科研人员提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：GH Ab
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：2 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Developmental Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human anti-GH antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 82-105 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 84-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 82-105 | |||||
回收率：
| The recovery of human anti-GH antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 82-105 | ||||
| EDTA plasma (n=4) | 93 | 84-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 5002.6042.5732.5892.448 2501.9942.1092.0521.911 1251.3821.3611.3721.231 62.50.8520.8450.8490.708 31.20.5240.5160.5200.379 15.60.3560.3680.3620.221 7.80.2420.2530.2480.107 00.1450.1360.141 | ||||||
货期：3-5 working days
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