| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RPB5; XAP4; RPABC1; hRPB25; hsRPB5 |
| Entrez GeneID | 5434 |
| WB Predicted band size | Calculated MW: 25 kDa; Observed MW: 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human POLR2E |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于RNA Polymerases I/II/III Subunit ABC1抗体的3篇文献示例(注：内容为模拟，实际文献需根据具体研究验证)：
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1. **文献名称**: "Structural and functional analysis of the human RNA polymerase II subunit ABC1"
**作者**: Smith J et al.
**摘要**: 该研究通过免疫沉淀和质谱技术鉴定了人RNA聚合酶II的ABC1亚基(POLR2K)，并制备了特异性抗体。抗体用于Western blot和免疫荧光实验，证实ABC1在三种RNA聚合酶复合物中普遍存在，并参与转录起始复合物的组装。
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2. **文献名称**: "Role of ABC1 subunit in RNA polymerase III transcription regulation"
**作者**: Chen L et al.
**摘要**: 本文利用ABC1亚基抗体进行ChIP-seq分析，揭示了该亚基在RNA聚合酶III介导的tRNA基因转录中的调控作用。实验表明，ABC1缺失会显著降低Pol III的转录活性，提示其在基因表达调控中的关键地位。
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3. **文献名称**: "Antibody-based profiling of RNA polymerase subunits in cancer cells"
**作者**: Wang Y et al.
**摘要**: 研究团队开发了一种针对ABC1亚基的高特异性单克隆抗体，用于检测多种癌细胞系中RNA聚合酶I/II/III的表达水平。结果显示，ABC1在快速增殖的肿瘤细胞中高表达，可能作为潜在的治疗靶点。
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(注：若需真实文献，建议通过PubMed搜索关键词如“POLR2K antibody”或“RNA Polymerase common subunit ABC1”获取最新研究。)
RNA Polymerases I/II/III Subunit ABC1 antibodies target a conserved subunit shared by all three eukaryotic RNA polymerases (Pol I, II, and III), which are essential for transcription. The ABC1 subunit (also referred to as ABC10. RPB12. or RPC10. depending on the polymerase) is a small, highly conserved protein critical for enzyme assembly and stability. In Pol II, ABC1 interacts with other core subunits to form the catalytic center, supporting transcription initiation and elongation. Its structural and functional conservation across Pol I, II, and III highlights its fundamental role in ribosomal RNA (Pol I), mRNA (Pol II), and tRNA/small RNA (Pol III) synthesis.
Antibodies against ABC1 are valuable tools for studying transcriptional regulation, polymerase complex composition, and subcellular localization. They enable detection of all three polymerases in techniques like Western blotting, immunoprecipitation, or immunofluorescence. Researchers use these antibodies to explore dysregulated transcription in diseases such as cancer, developmental disorders, or viral infections that hijack host machinery. Specificity validation is crucial due to shared epitopes across polymerases, and controls (e.g., siRNA knockdown) are often included to confirm target identity. ABC1 antibodies thus bridge molecular biology and disease research, offering insights into transcriptional mechanisms.
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