Human Triosephosphate isomerase(TPI1) ELISA kit

大包装询价

货号: IPD-EL024102HU

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人磷酸丙糖异构酶(TPI1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TPI1含量。TPI1即丙糖磷酸异构酶1，是糖酵解途径关键酶。它参与细胞能量代谢，为细胞增殖、生长提供能量。在肿瘤研究中，TPI1表达异常与肿瘤发生发展相关，可影响肿瘤细胞糖代谢和生物学行为，是潜在肿瘤治疗靶点，研究其机制或助抗癌药开发。试剂盒检测范围为28 pg/mL-1800 pg/mL，适用于体外研究TPI1在细胞代谢调控、疾病模型构建及药物干预实验中的动态变化。其宽泛的检测范围和多样化的样本兼容性，可满足科研人员对生物体内TPI1表达水平的精准分析需求，为探索代谢相关疾病的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
TPI1 ELISA Kit; TPI ELISA Kit; Triosephosphate isomerase ELISA Kit; TIM ELISA Kit; EC 5.3.1.1 ELISA Kit; Methylglyoxal synthase ELISA Kit; EC 4.2.3.3 ELISA Kit; Triose-phosphate isomerase ELISA Kit
缩写：
TPI1
Uniprot No.：
P60174
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates, cell lysates
检测范围：
28 pg/mL-1800 pg/mL
灵敏度：
7 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Metabolism
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TPI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	106
	Range %	100-112
1:2	Average %	95
	Range %	92-99
1:4	Average %	90
	Range %	86-96
1:8	Average %	93
	Range %	90-97

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
1800	2.377	2.433	2.405	2.308
900	1.646	1.774	1.710	1.613
450	1.312	1.285	1.299	1.202
225	0.857	0.922	0.890	0.793
112.5	0.465	0.470	0.468	0.371
56	0.322	0.300	0.311	0.214
28	0.203	0.204	0.204	0.107
0	0.094	0.099	0.097

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.