Recombinant Human UBE2Z protein

中文名： 泛素偶联酶E2Z(UBE2Z)重组蛋白
别名： UBE2Z；Ubiquitin-conjugating enzyme E2 Z

货号: PA1000-3386

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>85%SDS-PAGE.
种属	Human
靶点	UBE2Z
Uniprot No	Q9H832
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	109-354aa
氨基酸序列	MSIYKEPPPGMFVVPDTVDMTKIHALITGPFDTPYEGGFFLFVFRCPPDY PIHPPRVKLMTTGNNTVRFNPNFYRNGKVCLSILGTWTGPAWSPAQSISS VLISIQSLMTENPYHNEPGFEQERHPGDSKNYNECIRHETIRVAVCDMME GKCPCPEPLRGVMEKSFLEYYDFYEVACKDRLHLQGQTMQDPFGEKRGHF DYQSLLMRLGLIRQKVLERLHNENAEMDSDSSSSGTETDLHGSLRV
预测分子量	30 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于UBE2Z重组蛋白的3篇参考文献及其摘要概括：

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1. **文献名称**: "UBE2Z is a critical component of the NF-κB signaling pathway promoting tumor cell migration and invasion"

**作者**: Li Y, et al.

**摘要**: 该研究探讨了UBE2Z在NF-κB信号通路中的作用，发现其通过泛素化修饰关键调控蛋白(如IKK复合物)促进肿瘤细胞的迁移和侵袭。研究利用重组UBE2Z蛋白进行体外泛素化实验，证实其直接参与炎症和癌症相关通路的调控。

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2. **文献名称**: "Structural and functional characterization of the ubiquitin-conjugating enzyme UBE2Z"

**作者**: Smith J, et al.

**摘要**: 本研究通过X射线晶体学解析了UBE2Z重组蛋白的三维结构，揭示了其催化活性位点和与泛素结合的结构域。实验表明UBE2Z在底物识别中具有独特的泛素链连接能力，可能参与K11-linked泛素链的形成。

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3. **文献名称**: "UBE2Z-mediated ubiquitination regulates the stability of the BRCA1-BARD1 complex in DNA repair"

**作者**: Wang H, et al.

**摘要**: 该文献报道UBE2Z通过重组蛋白实验证明其与BRCA1-BARD1复合物相互作用，并调控该复合物的稳定性以影响DNA损伤修复。研究发现UBE2Z的缺失会导致同源重组修复效率下降，提示其在基因组稳定性中的重要性。

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**备注**：以上文献信息为模拟生成，实际文献需通过PubMed、Google Scholar等平台检索确认。若需具体文献，建议结合关键词“UBE2Z recombinant protein”“ubiquitination mechanism”进一步筛选。

背景信息

UBE2Z (Ubiquitin-Conjugating Enzyme E2 Z) is a member of the E2 ubiquitin-conjugating enzyme family, which plays a critical role in the ubiquitin-proteasome system (UPS) responsible for targeted protein degradation. Encoded by the UBE2Z gene located on human chromosome 17q21.32. this enzyme facilitates the transfer of ubiquitin to substrate proteins, a process essential for regulating cellular processes such as protein turnover, DNA repair, and signal transduction. UBE2Z contains a conserved catalytic core domain typical of E2 enzymes and a unique N-terminal UBA domain that may mediate interactions with ubiquitin or other regulatory proteins.

Recombinant UBE2Z protein is typically produced in bacterial or mammalian expression systems, enabling researchers to study its biochemical properties and functional mechanisms in vitro. Its recombinant form is crucial for elucidating its role in forming Lys-48-linked polyubiquitin chains, which tag proteins for proteasomal degradation. Studies suggest UBE2Z interacts with specific E3 ubiquitin ligases, such as the linear ubiquitin chain assembly complex (LUBAC), to modulate NF-κB signaling and inflammatory responses. Dysregulation of UBE2Z has been implicated in diseases like cancer, neurodegenerative disorders, and immune dysregulation, highlighting its therapeutic potential.

Research applications include enzyme activity assays, protein interaction studies, and structural analysis to design inhibitors targeting the UPS. Recombinant UBE2Z also serves as a tool to explore post-translational modifications and substrate specificity, advancing our understanding of ubiquitination dynamics in health and disease.