首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠转化生长因子β1(TGF-β1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TGFB1含量。TGFB1 是重要的细胞因子，在胚胎发育、组织修复等生理过程中发挥关键作用。其异常表达与肿瘤、纤维化等疾病密切相关。研究机制主要围绕其信号通路展开，通过调控下游基因表达影响细胞增殖、分化、凋亡等，为疾病治疗提供潜在靶点。试剂盒检测范围为6.25 pg/mL-400 pg/mL，其动态监测对炎症性疾病、肿瘤微环境及再生医学等研究具有重要意义；尤其适用于动态监测实验动物模型（如肝纤维化、肺损伤或肿瘤移植模型）中TGF-β1的分泌变化，或体外细胞实验（如巨噬细胞极化、干细胞分化等）的因子表达水平分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tgfb1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
缩写：TGFB1
Uniprot No.：P17246
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 89-99 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 91-101 | |||||
| 1:800 | Average % | 102 | ||||
| Range % | 98-107 | |||||
回收率：
| The recovery of rat TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-104 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 4002.5602.5892.5752.388 2002.2622.2992.2812.094 1001.7871.7981.7931.606 501.0981.0761.0870.900 250.5810.5980.5900.403 12.50.4210.4520.4370.250 6.250.3070.3210.3140.127 00.1860.1880.187 | ||||||
货期：3-5 working days
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