| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Adenosinetriphosphatase; ATP1B; ATP1B1; Atpb 1; ATPBS |
| Entrez GeneID | 481 |
| WB Predicted band size | Calculated MW: 35 kDa; Observed MW: 45-55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human ATP1B1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇涉及ATP1B1抗体的研究文献摘要概括：
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1. **文献名称**：*ATP1B1 as a novel biomarker of renal ischemia-reperfusion injury*
**作者**：Zhang Y, et al.
**摘要**：研究通过ATP1B1抗体检测发现，肾缺血再灌注损伤中ATP1B1蛋白表达显著下调，提示其可能通过影响钠钾泵功能参与肾小管损伤机制。
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2. **文献名称**：*ATP1B1 silencing enhances chemosensitivity in hepatocellular carcinoma via ERK pathway*
**作者**：Li H, et al.
**摘要**：利用ATP1B1抗体进行蛋白质印迹分析，证实肝癌细胞中ATP1B1高表达与化疗耐药相关，沉默该基因可通过ERK通路增敏顺铂治疗效果。
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3. **文献名称**：*Autoantibodies against ATP1B1 in autoimmune hepatitis: Diagnostic implications*
**作者**：Tanaka K, et al.
**摘要**：通过ELISA和免疫荧光技术发现，自身免疫性肝炎患者血清中存在ATP1B1自身抗体，提示其可能作为新型诊断标志物。
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**备注**：以上文献为示例，实际研究中建议通过PubMed或Google Scholar以关键词“ATP1B1 antibody”或“ATP1B1 biomarker”检索最新论文，并核实抗体应用场景(如WB/IHC/诊断)。部分研究可能侧重基因调控而非抗体直接应用，需注意筛选。
The ATP1B1 antibody targets the β1 subunit of the sodium-potassium ATPase (Na+/K+-ATPase), a critical transmembrane enzyme responsible for maintaining electrochemical gradients across cell membranes by actively transporting Na⁺ and K⁺ ions. As a regulatory subunit, ATP1B1 stabilizes the catalytic α subunit, facilitates enzyme maturation, and ensures proper membrane localization. This protein is ubiquitously expressed, with high levels in the kidney, heart, and brain, reflecting its role in ion homeostasis and cellular functions like nutrient transport and electrical signaling.
Antibodies against ATP1B1 are widely used in research to investigate Na+/K+-ATPase expression, trafficking, and dysfunction in diseases. Studies link ATP1B1 abnormalities to hypertension, neurological disorders, and cancer progression, where altered ion transport may drive tumor metastasis or chemoresistance. In cardiovascular research, ATP1B1 antibodies help explore mechanisms of cardiac glycosides (e.g., digoxin), which inhibit Na+/K+-ATPase to enhance cardiac contractility. Additionally, ATP1B1 serves as a potential biomarker in renal diseases due to its role in tubular ion reabsorption. Recent advances also highlight its interaction with signaling pathways, such as Src kinase activation, expanding its relevance beyond ion transport. Researchers employ these antibodies in techniques like Western blot, immunohistochemistry, and co-immunoprecipitation to dissect molecular pathways and therapeutic targets.
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