首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血清淀粉样蛋白A(SAA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SAA含量。试剂盒检测范围为78 ng/mL-5000 ng/mL，适用于炎症机制研究、感染性疾病模型评估、免疫调节药物开发等科研领域，例如在实验性关节炎、脓毒症或肿瘤模型中动态监测SAA水平变化，为病理机制解析及治疗干预效果评价提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：SAA
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：78 ng/mL-5000 ng/mL
灵敏度：19.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 93-99 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-97 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 99-105 | |||||
回收率：
| The recovery of rat SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 82-91 | ||||
| EDTA plasma (n=4) | 98 | 95-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected50001.9781.9981.9881.84625001.3411.3981.3701.22812500.8130.8340.8240.6826250.4760.4980.4870.345312.50.3080.3280.3180.176156.250.2450.2580.2520.110780.2020.2150.2090.06700.1410.1430.142 | ||||||
货期：3-5 working days
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