首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠S100-A9蛋白(S100A9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的S100A9含量。S100A9是一个重要靶点。它属于S100蛋白家族，在多种细胞中表达。其背景与炎症反应、肿瘤发生发展等密切相关。研究机制方面，它可参与调节细胞的增殖、迁移等过程，还能影响免疫细胞功能，是当前疾病治疗研究的热点之一。试剂盒检测范围为0.45 ng/mL-30 ng/mL，适用于科研领域研究炎症相关疾病模型（如关节炎、脑损伤或肿瘤模型）中S100A9的动态表达，分析其作为炎症标志物的生物学功能。为探索S100A9在病理生理过程中的作用机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：S100a9 ELISA kit; Mrp14Protein S100-A9 ELISA kit; Calgranulin-B ELISA kit; Migration inhibitory factor-related protein 14 ELISA kit; MRP-14 ELISA kit; p14 ELISA kit; Myeloid-related protein 14 ELISA kit; S100 calcium-binding protein A9 ELISA kit
缩写：S100A9
Uniprot No.：P50116
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.45 ng/mL-30 ng/mL
灵敏度：0.11 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat S100A9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
| 1:2 | Average % | 100 | |||||
| Range % | 95-106 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
| 1:8 | Average % | 96 | |||||
| Range % | 91-100 | ||||||
回收率：
| The recovery of rat S100A9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-100 | |||||
| EDTA plasma (n=4) | 84 | 80-88 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected302.1052.0352.0701.895151.6261.5261.5761.4017.51.0131.0591.0360.8613.750.6740.7020.6880.5131.80.5020.5170.5100.3350.90.3710.3940.3830.2080.450.2550.2410.2480.07300.1720.1780.175 | |||||||
货期：3-5 working days
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