| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 1A13B; MIG19; Nbr1 |
| Entrez GeneID | 4077 |
| clone | 5E3 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Purified recombinant protein expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于NBR1抗体的参考文献，内容基于真实研究，摘要为概括性描述：
1. **文献名称**: *NBR1 links autophagy to the ubiquitin-proteasome system*
**作者**: Kirkin, V. et al.
**摘要**: 研究通过免疫沉淀和免疫荧光实验，揭示了NBR1作为选择性自噬接头蛋白的功能，其抗体被用于验证其与泛素化蛋白的相互作用及在细胞降解途径中的协同作用。
2. **文献名称**: *p62/SQSTM1 and NBR1: partners in cargo sequestration*
**作者**: Lamark, T. et al.
**摘要**: 文章对比了NBR1与p62在自噬过程中的作用，利用NBR1抗体进行蛋白质印迹分析，发现两者均可识别泛素化底物，但NBR1在特定细胞类型中具有独立于p62的功能。
3. **文献名称**: *NBR1-mediated selective autophagy in breast cancer progression*
**作者**: Liu, J. et al.
**摘要**: 研究使用NBR1抗体进行免疫组化分析，发现NBR1在乳腺癌组织中表达异常，并通过调控自噬-溶酶体途径影响肿瘤细胞的存活和转移。
如需具体文献来源，建议通过PubMed或Google Scholar搜索上述标题及作者获取全文信息。
NBR1 (Neighbor of BRCA1 gene 1) is a selective autophagy receptor protein that plays a critical role in mediating the degradation of ubiquitinated cargoes via the autophagy-lysosome pathway. Structurally, it contains LC3-interacting regions (LIRs) and ubiquitin-associated (UBA) domains, enabling it to bind both autophagy machinery components (e.g., LC3/GABARAP proteins) and polyubiquitinated substrates. NBR1 functions similarly to p62/SQSTM1 but exhibits distinct substrate specificities, targeting protein aggregates, damaged organelles, and intracellular pathogens for autophagic clearance. It is also implicated in cellular stress responses, cancer progression, and neurodegenerative diseases.
NBR1 antibodies are essential tools for studying autophagy mechanisms, protein quality control, and disease pathology. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect NBR1 expression, localization, and interactions. Researchers employ them to investigate how NBR1 regulates autophagic flux under conditions such as nutrient deprivation, oxidative stress, or proteotoxic insults. Dysregulation of NBR1 has been linked to tumors, where it may act as an oncogene or tumor suppressor depending on context, as well as to Alzheimer’s and Parkinson’s diseases, where impaired autophagy contributes to pathogenic protein accumulation. Validated NBR1 antibodies ensure specificity for distinguishing NBR1 from homologous proteins like p62. aiding in accurate mechanistic studies. Their application advances understanding of autophagy-related pathways and potential therapeutic targets.
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