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产品描述：人再生胰岛衍生1α/胰石蛋白/胰线蛋白(REG1A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的REG1A含量。REG1A作为一个靶点，在人体生理和病理过程中扮演重要角色。其研究机制主要围绕它在细胞信号传导、增殖与凋亡等环节的作用展开，探索它与疾病发生发展的关联，为开发针对性的治疗策略，如药物研发等提供理论基础。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本产品为科研人员研究胰腺相关疾病的分子机制、探索REG1A在代谢调控或肿瘤微环境中的作用提供可靠工具，尤其适用于体外检测血清/血浆样本中REG1A的浓度动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ICRF ELISA Kit; Islet cells regeneration factor ELISA Kit; Islet of Langerhans regenerating protein ELISA Kit; Lithostathine 1 alpha ELISA Kit; Lithostathine 1 alpha precursor ELISA Kit; Lithostathine ELISA Kit; Lithostathine-1-alpha ELISA Kit; MGC12447 ELISA Kit; P19 ELISA Kit; Pancreatic stone protein ELISA Kit; Pancreatic stone protein secretory ELISA Kit; Pancreatic thread protein ELISA Kit; Protein X ELISA Kit; PSP ELISA Kit; PSPS ELISA Kit; PSPS1 ELISA Kit; PTP ELISA Kit; RATRGPI ELISA Kit; REG ELISA Kit; REG-1-alpha ELISA Kit; Reg1 ELISA Kit; REG1A ELISA Kit; REG1A_HUMAN ELISA Kit; Regenerating islet derived 1 alpha ELISA Kit; Regenerating islet derived 1 alpha pancreatic stone protein pancreatic thread protein ELISA Kit; Regenerating islet-derived protein 1-alpha ELISA Kit; Regenerating protein I alpha ELISA Kit
缩写：REG1A
Uniprot No.：P05451
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human REG1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 83-97 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of human REG1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-102 | ||||
| EDTA plasma (n=4) | 102 | 96-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.6082.5142.5612.42651.8931.7871.8401.7052.51.1471.0851.1160.9811.250.6270.6070.6170.4820.6250.3740.3670.3710.2360.3120.2870.2930.2900.1550.1560.2310.2260.2290.09400.1360.1340.135 | ||||||
货期：3-5 working days
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